Immunocytochemical identification of proliferating cell types in mouse mammary gland

A. Sapino, L. Macri, P. Gugliotta, G. Bussolati

Research output: Contribution to journalArticle

Abstract

To study cell proliferation in different cell types and segments of the mammary gland, we devised a dual staining procedure, combining nuclear labeling by 5-bromo-2'-deoxyuridine (BrdU) uptake (revealed by a dark-brown precipitate) and an alternative (red or blue) cytoplasmic labeling by antibodies specific for the differentiation proteins of epithelial, myoepithelial, and secretory cell types. The following markers, revealed by APAAP or β-galactosidase procedure, were selected: α-smooth muscle actin for the myoepithelial cells, keratin (detected by AE1 monoclonal) for the luminal epithelial cells, α-lactalbumin and β-casein for the secretory cells. To follow the full process of organogenesis, the study was conducted in mouse mammary glands from virgin, primed, and lactating animals and from glands cultured in vitro under specific hormone stimulation. Cell proliferation was localized mainly in focal areas (end buds), and mostly corresponded to 'null' undifferentiated cells. Estrogen and progestin stimulation induced a relative increase of proliferating differentiated cells of either epithelial or myoepithelial type, localized in ducts and alveolar structures. Prolactin stimulation induced proliferation in secretory cells.

Original languageEnglish
Pages (from-to)1541-1547
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume38
Issue number11
Publication statusPublished - 1990

Fingerprint

Human Mammary Glands
Epithelial Cells
Cell Proliferation
Galactosidases
Lactalbumin
Null Lymphocytes
Organogenesis
Progestins
Bromodeoxyuridine
Keratins
Caseins
Prolactin
Smooth Muscle
Actins
Estrogens
Hormones
Staining and Labeling
Antibodies
Proteins

Keywords

  • 5-Bromo-2'-deoxyuridine
  • Cell differentiation
  • Immunocytochemistry
  • Mammary gland
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Immunocytochemical identification of proliferating cell types in mouse mammary gland. / Sapino, A.; Macri, L.; Gugliotta, P.; Bussolati, G.

In: Journal of Histochemistry and Cytochemistry, Vol. 38, No. 11, 1990, p. 1541-1547.

Research output: Contribution to journalArticle

Sapino, A. ; Macri, L. ; Gugliotta, P. ; Bussolati, G. / Immunocytochemical identification of proliferating cell types in mouse mammary gland. In: Journal of Histochemistry and Cytochemistry. 1990 ; Vol. 38, No. 11. pp. 1541-1547.
@article{ecbf525a8600448cbf797285639b310d,
title = "Immunocytochemical identification of proliferating cell types in mouse mammary gland",
abstract = "To study cell proliferation in different cell types and segments of the mammary gland, we devised a dual staining procedure, combining nuclear labeling by 5-bromo-2'-deoxyuridine (BrdU) uptake (revealed by a dark-brown precipitate) and an alternative (red or blue) cytoplasmic labeling by antibodies specific for the differentiation proteins of epithelial, myoepithelial, and secretory cell types. The following markers, revealed by APAAP or β-galactosidase procedure, were selected: α-smooth muscle actin for the myoepithelial cells, keratin (detected by AE1 monoclonal) for the luminal epithelial cells, α-lactalbumin and β-casein for the secretory cells. To follow the full process of organogenesis, the study was conducted in mouse mammary glands from virgin, primed, and lactating animals and from glands cultured in vitro under specific hormone stimulation. Cell proliferation was localized mainly in focal areas (end buds), and mostly corresponded to 'null' undifferentiated cells. Estrogen and progestin stimulation induced a relative increase of proliferating differentiated cells of either epithelial or myoepithelial type, localized in ducts and alveolar structures. Prolactin stimulation induced proliferation in secretory cells.",
keywords = "5-Bromo-2'-deoxyuridine, Cell differentiation, Immunocytochemistry, Mammary gland, Proliferation",
author = "A. Sapino and L. Macri and P. Gugliotta and G. Bussolati",
year = "1990",
language = "English",
volume = "38",
pages = "1541--1547",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "11",

}

TY - JOUR

T1 - Immunocytochemical identification of proliferating cell types in mouse mammary gland

AU - Sapino, A.

AU - Macri, L.

AU - Gugliotta, P.

AU - Bussolati, G.

PY - 1990

Y1 - 1990

N2 - To study cell proliferation in different cell types and segments of the mammary gland, we devised a dual staining procedure, combining nuclear labeling by 5-bromo-2'-deoxyuridine (BrdU) uptake (revealed by a dark-brown precipitate) and an alternative (red or blue) cytoplasmic labeling by antibodies specific for the differentiation proteins of epithelial, myoepithelial, and secretory cell types. The following markers, revealed by APAAP or β-galactosidase procedure, were selected: α-smooth muscle actin for the myoepithelial cells, keratin (detected by AE1 monoclonal) for the luminal epithelial cells, α-lactalbumin and β-casein for the secretory cells. To follow the full process of organogenesis, the study was conducted in mouse mammary glands from virgin, primed, and lactating animals and from glands cultured in vitro under specific hormone stimulation. Cell proliferation was localized mainly in focal areas (end buds), and mostly corresponded to 'null' undifferentiated cells. Estrogen and progestin stimulation induced a relative increase of proliferating differentiated cells of either epithelial or myoepithelial type, localized in ducts and alveolar structures. Prolactin stimulation induced proliferation in secretory cells.

AB - To study cell proliferation in different cell types and segments of the mammary gland, we devised a dual staining procedure, combining nuclear labeling by 5-bromo-2'-deoxyuridine (BrdU) uptake (revealed by a dark-brown precipitate) and an alternative (red or blue) cytoplasmic labeling by antibodies specific for the differentiation proteins of epithelial, myoepithelial, and secretory cell types. The following markers, revealed by APAAP or β-galactosidase procedure, were selected: α-smooth muscle actin for the myoepithelial cells, keratin (detected by AE1 monoclonal) for the luminal epithelial cells, α-lactalbumin and β-casein for the secretory cells. To follow the full process of organogenesis, the study was conducted in mouse mammary glands from virgin, primed, and lactating animals and from glands cultured in vitro under specific hormone stimulation. Cell proliferation was localized mainly in focal areas (end buds), and mostly corresponded to 'null' undifferentiated cells. Estrogen and progestin stimulation induced a relative increase of proliferating differentiated cells of either epithelial or myoepithelial type, localized in ducts and alveolar structures. Prolactin stimulation induced proliferation in secretory cells.

KW - 5-Bromo-2'-deoxyuridine

KW - Cell differentiation

KW - Immunocytochemistry

KW - Mammary gland

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=0025149620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025149620&partnerID=8YFLogxK

M3 - Article

C2 - 2212615

AN - SCOPUS:0025149620

VL - 38

SP - 1541

EP - 1547

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 11

ER -