Immunocytochemical localization of D-amino acid oxidase in rat brain

Sandra Moreno, Roberta Nardacci, Annamaria Cimini, Maria Paola Cerù

Research output: Contribution to journalArticle

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Abstract

D-amino acid oxidase (D-AAO) is a peroxisomal flavoenzyme, the physiological substrate and the precise function of which are still unclear. We have investigated D-AAO distribution in rat brain, by immunocytochemistry, with an affinity-purified polyclonal antibody. Immunoreactivity occurred in both neuronal and glial cells, albeit at different densities. Glial immunostaning was strongest in the caudal brainstem and cerebellar cortex, particularly in astrocytes, Golgi-Bergmann glia, and tanycytes. Hindbrain neurons were generally more immunoreactive than those in the forebrain. Immunopositive forebrain cell populations included mitral cells in the olfactory bulb, cortical and hippocampal neurons, ventral pallidum, and septal, reticular thalamic, and paraventricular hypothalamic nuclei. Within the positive regions, not all the neuronal populations were equally immunoreactive; for example, in the thalamus, only the reticular and anterodorsal nuclei showed intense labelling. In the hindbrain, immunopositivity was virtually ubiquitous, and was especially strong in the reticular formation, pontine, ventral and dorsal cochlear, vestibular, cranial motor nuclei, deep cerebellar nuclei, and the cerebellar cortex, especially in Golgi and Purkinje cells.

Original languageEnglish
Pages (from-to)169-185
Number of pages17
JournalJournal of Neurocytology
Volume28
Issue number3
DOIs
Publication statusPublished - 1999

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D-Amino-Acid Oxidase
Neuroglia
Rhombencephalon
Cerebellar Cortex
Prosencephalon
Brain
Anterior Thalamic Nuclei
Ependymoglial Cells
Neurons
Cerebellar Nuclei
Paraventricular Hypothalamic Nucleus
Olfactory Bulb
Purkinje Cells
Cochlea
Thalamus
Astrocytes
Population
Brain Stem
Immunohistochemistry
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)
  • Anatomy
  • Cell Biology
  • Histology

Cite this

Immunocytochemical localization of D-amino acid oxidase in rat brain. / Moreno, Sandra; Nardacci, Roberta; Cimini, Annamaria; Cerù, Maria Paola.

In: Journal of Neurocytology, Vol. 28, No. 3, 1999, p. 169-185.

Research output: Contribution to journalArticle

Moreno, Sandra ; Nardacci, Roberta ; Cimini, Annamaria ; Cerù, Maria Paola. / Immunocytochemical localization of D-amino acid oxidase in rat brain. In: Journal of Neurocytology. 1999 ; Vol. 28, No. 3. pp. 169-185.
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