Immunocytochemical localization of vinculin in muscle and nerve

R. Massa, G. Silvestri, G. Sancesario, G. Bernardi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A complete survey of the immunofluorescence distribution of the cytoskeletal protein vinculin in the normal skeletal muscle and peripheral nerve of humans and of different rodent species was performed. Our results enable us to localize vinculin in different types of adhesion plaques such as sarcolemmal costameres and neuromuscular and myotendinous junctions, but also in a fine intermyofibrillar lattice, possibly associated with intermediate filaments and/or with the triads. Moreover, we describe the presence of vinculin in junctional domains of several, previously unrecognized, specialized cells such as: the outer sheath of the muscle spindle capsule, the multilayered flat cells of the perineurium, the smooth muscle cells of epineurial blood vessels, and the endothelial cells in the endoneurium. These data call for a major role of vinculin in mechanisms of adhesion between cells, between cell and substrate and between intermyofibrillar components in the neuromuscular system. Such knowledge provides an anatomical background for studies on the possible pathological effects induced by an impairment in vinculin function.

Original languageEnglish
Pages (from-to)1277-1284
Number of pages8
JournalMuscle and Nerve
Volume18
Issue number11
DOIs
Publication statusPublished - 1995

Fingerprint

Vinculin
Muscles
Peripheral Nerves
Costameres
Muscle Spindles
Focal Adhesions
Cytoskeletal Proteins
Intermediate Filaments
Neuromuscular Junction
Cell Adhesion
Smooth Muscle Myocytes
Capsules
Fluorescent Antibody Technique
Blood Vessels
Rodentia
Skeletal Muscle
Endothelial Cells

Keywords

  • immunofluorescence
  • muscle spindle
  • nerve
  • skeletal muscle
  • smooth muscle
  • vinculin

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Massa, R., Silvestri, G., Sancesario, G., & Bernardi, G. (1995). Immunocytochemical localization of vinculin in muscle and nerve. Muscle and Nerve, 18(11), 1277-1284. https://doi.org/10.1002/mus.880181110

Immunocytochemical localization of vinculin in muscle and nerve. / Massa, R.; Silvestri, G.; Sancesario, G.; Bernardi, G.

In: Muscle and Nerve, Vol. 18, No. 11, 1995, p. 1277-1284.

Research output: Contribution to journalArticle

Massa, R, Silvestri, G, Sancesario, G & Bernardi, G 1995, 'Immunocytochemical localization of vinculin in muscle and nerve', Muscle and Nerve, vol. 18, no. 11, pp. 1277-1284. https://doi.org/10.1002/mus.880181110
Massa, R. ; Silvestri, G. ; Sancesario, G. ; Bernardi, G. / Immunocytochemical localization of vinculin in muscle and nerve. In: Muscle and Nerve. 1995 ; Vol. 18, No. 11. pp. 1277-1284.
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