Immunocytochemical location of hydroxyproline rich glycoproteins at the interface between a mycorrhizal fungus and its host plants

P. Bonfante-Fasolo, L. Tamagnone, R. Peretto, M. T. Esquerré-Tugayé, D. Mazau, M. Mosiniak, B. Vian

Research output: Contribution to journalArticlepeer-review

Abstract

When vesicular-arbuscular mycorrhizal (VAM) fungi colonize the cortical cells of their host plant roots, the hyphae are separated from the host cytoplasm by the invaginated host plasmalemma and interfacial material. The presence of hydroxyproline rich glycoproteins (HRGPs) at the interface was investigated with a polyclonal antibody obtained against melon callus HRGP2b. By using a combination of cytochemical methods, antigens were detected in pea, in both the presence and absence of Glomus versiforme, a mycorrhizal fungus. For comparison, observations were performed in parallel with leek as a monocot host. Antigens were localized over the pea cell wall in root tissues. At the ultrastructural level, gold granules were mostly present in the periplasmic space. In mycorrhizal plants, the most substantial deposition occurred at the interface between the fungal wall and the host membrane. Dot blot experiments revealed HRGP2b antigens in soluble root fractions from both uninfected and mycorrhizal samples. The results demonstrate that HRGP2b antigens can be localized over the cell wall of both dicot and monocot hosts, although they mostly occur in the contact zone in infected samples. Their presence-in the company of localized glucans and pectins-means that the contact zone can be regarded as an apoplastic space presenting a structural response to the symbiotic mycorrhizal status.

Original languageEnglish
Pages (from-to)127-138
Number of pages12
JournalProtoplasma
Volume165
Issue number1-3
DOIs
Publication statusPublished - Feb 1991

Keywords

  • Cell wall
  • Hydroxyproline rich glycoproteins
  • Interface zone
  • Leek
  • Mycorrhizal fungi
  • Pea

ASJC Scopus subject areas

  • Cell Biology
  • Plant Science

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