Immunocytological identification of the microsomal calcium store of nonmuscle cells

Tullio Pozzan, Pompeo Volpe, Francesco Zorzato, Mariangela Bravin, Daniel P. Lew, Karl H. Krause, Sadamitsu Hashimoto, Bernardino Bruno, Jacopo Meldolesi

Research output: Contribution to journalArticle

Abstract

The biochemical and functional similarities between skeletal muscle sarcoplasmic reticulum and the microsomal Ca2+ store of nonmuscle cells are discussed. It is shown that antibodies raised against two characteristic proteins of sarcoplasmic reticulum, Ca2+ ATPase and calsequestrin, recognize similar proteins in nonmuscle cells. The subcellular distribution of these two antigens was studied at the subcellular levels in ultrathin cryosections. In a variety of cell types these two proteins were found to be localized in small membrane enclosed vesicles, apparently distinct from other known organelles. We propose that these newly recognized structures (calciosomes) represent the functional equivalent of sarcoplasmic reticulum in nonmuscle cells.

Original languageEnglish
Pages (from-to)S80-S84
JournalJournal of Cardiovascular Pharmacology
Volume12
Issue number5
Publication statusPublished - 1988

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Keywords

  • Ca<sup>2+</sup>
  • Ca<sup>2+</sup> binding protein
  • InsP3

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Pozzan, T., Volpe, P., Zorzato, F., Bravin, M., Lew, D. P., Krause, K. H., Hashimoto, S., Bruno, B., & Meldolesi, J. (1988). Immunocytological identification of the microsomal calcium store of nonmuscle cells. Journal of Cardiovascular Pharmacology, 12(5), S80-S84.