Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization

Cristina Manferdini; Nicoletta Zini; Elena Gabusi; Francesca Paolella; Elisabetta Lambertini; Letizia Penolazzi; Roberta Piva; Gina Lisignoli

doi:10.1080/03008207.2017.1408600

Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization

Cristina Manferdini, Nicoletta Zini, Elena Gabusi, Francesca Paolella, Elisabetta Lambertini, Letizia Penolazzi, Roberta Piva, Gina Lisignoli

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).

MATERIAL AND METHODS: hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.

RESULTS: Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.

CONCLUSIONS: We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.

Original language	English
Pages (from-to)	42-45
Number of pages	4
Journal	Connective Tissue Research
Volume	59
Issue number	sup1
DOIs	https://doi.org/10.1080/03008207.2017.1408600
Publication status	Published - Dec 2018

Keywords

Collagen type XV
Human mesenchymal stem cells
immunogold
mineralization
osteogenesis

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10.1080/03008207.2017.1408600

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@article{bab7166bbac74ebb8916d76aa3e06684,

title = "Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization",

abstract = "Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).MATERIAL AND METHODS: hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.RESULTS: Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.CONCLUSIONS: We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.",

keywords = "Collagen type XV, Human mesenchymal stem cells, immunogold, mineralization, osteogenesis",

author = "Cristina Manferdini and Nicoletta Zini and Elena Gabusi and Francesca Paolella and Elisabetta Lambertini and Letizia Penolazzi and Roberta Piva and Gina Lisignoli",

year = "2018",

month = dec,

doi = "10.1080/03008207.2017.1408600",

language = "English",

volume = "59",

pages = "42--45",

journal = "Connective Tissue Research",

issn = "0300-8207",

publisher = "Informa Healthcare",

number = "sup1",

}

TY - JOUR

T1 - Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization

AU - Manferdini, Cristina

AU - Zini, Nicoletta

AU - Gabusi, Elena

AU - Paolella, Francesca

AU - Lambertini, Elisabetta

AU - Penolazzi, Letizia

AU - Piva, Roberta

AU - Lisignoli, Gina

PY - 2018/12

Y1 - 2018/12

N2 - Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).MATERIAL AND METHODS: hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.RESULTS: Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.CONCLUSIONS: We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.

AB - Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).MATERIAL AND METHODS: hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.RESULTS: Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.CONCLUSIONS: We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.

KW - Collagen type XV

KW - Human mesenchymal stem cells

KW - immunogold

KW - mineralization

KW - osteogenesis

U2 - 10.1080/03008207.2017.1408600

DO - 10.1080/03008207.2017.1408600

M3 - Article

C2 - 29745809

VL - 59

SP - 42

EP - 45

JO - Connective Tissue Research

JF - Connective Tissue Research

SN - 0300-8207

IS - sup1

ER -

Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization

Abstract

Keywords

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