Abstract
Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).
MATERIAL AND METHODS: hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.
RESULTS: Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.
CONCLUSIONS: We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.
Original language | English |
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Pages (from-to) | 42-45 |
Number of pages | 4 |
Journal | Connective Tissue Research |
Volume | 59 |
Issue number | sup1 |
DOIs | |
Publication status | Published - Dec 2018 |
Keywords
- Collagen type XV
- Human mesenchymal stem cells
- immunogold
- mineralization
- osteogenesis