Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization

Cristina Manferdini, Nicoletta Zini, Elena Gabusi, Francesca Paolella, Elisabetta Lambertini, Letizia Penolazzi, Roberta Piva, Gina Lisignoli

Research output: Contribution to journalArticle

Abstract

Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).

MATERIAL AND METHODS: hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.

RESULTS: Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.

CONCLUSIONS: We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.

Original languageEnglish
Pages (from-to)42-45
Number of pages4
JournalConnective Tissue Research
Volume59
Issue numbersup1
DOIs
Publication statusPublished - Dec 2018

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Keywords

  • Collagen type XV
  • Human mesenchymal stem cells
  • immunogold
  • mineralization
  • osteogenesis

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