Immunoelectron microscopy analysis of HCMV gpUL73 (gN) localization: Brief report

S. Pignatelli, P. Dal Monte, N. Zini, A. Valmori, N. M. Maraldi, M. P. Landini

Research output: Contribution to journalArticlepeer-review

Abstract

Human Cytomegalovirus (HCMV) UL73 encodes for a polymorphic structural glycoprotein, gpUL73(gN), conserved among herpesviruses. This study analyzed the intracellular and intraviral localization of gpUL73 by immuno-electron-microscopy comparing the reactivity of two different antibodies. We found that gN is an envelope component of the mature viral particle with at least a portion exposed at the virus surface and another at the internal side of the envelope. Furthermore, gpUL73 is also present in the matrix of dense bodies and "black holes". These results, as well as immunoblotting analysis, suggest that the two antibodies recognize different forms, fully processed or unprocessed, of gpUL73-gN.

Original languageEnglish
Pages (from-to)1247-1256
Number of pages10
JournalArchives of Virology
Volume147
Issue number6
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

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