Immunofiuorescent detection of factor VIII-related antigen in human platelets and megakaryocytes

Franco Piovella, E. Ascari, G. M. Sitar, G. D. Malamani, G. Cattaneo, E. Magliulo, E. Storti

Research output: Contribution to journalArticlepeer-review

Abstract

Platelets, lymphocytes, and megakaryocytes, specifically processed for the intracellular detection of antigens, were studied by indirect immunofluorescence techniques using monospecific antibodies directed against factor VUI-related antigen (F-VIII-RA). Specific staining was observed in platelets of the peripheral blood from normal individuals and from patients with haemophilia A, and in megakaryocytes and platelets of bone marrow from normal subjects. The same cells studied in patients with von Wille- brand’s disease were negative. No bone marrow biopsies were performed on patients with haemophilia A. Our results indicate that F-VIII-RA is localized in megakaryocytes and platelets of normal subjects; the possible role of this F-VIII-RA in platelet function is discussed.

Original languageEnglish
Pages (from-to)288-295
Number of pages8
JournalPathophysiology of Haemostasis and Thrombosis
Volume3
Issue number5-6
DOIs
Publication statusPublished - 1974

Keywords

  • Factor VIII-related antigen
  • Haemophilia A
  • immunofluorescence
  • Megakaryocytes
  • Platelets
  • von willebrand’s disease

ASJC Scopus subject areas

  • Hematology
  • Physiology (medical)

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