Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens

We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)₂ antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.