Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens

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Abstract

We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalApplied Pathology
Volume2
Issue number3
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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