Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens

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Abstract

We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalApplied Pathology
Volume2
Issue number3
Publication statusPublished - 1984

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Fluorescent Antibody Technique
Lymph Nodes
Bone Marrow
Kidney
Enzymes
Waldenstrom Macroglobulinemia
Paraffin
Immunoglobulin M
Immune Sera
Fluorescence
Biopsy
Membranes
hydroxyethyl methacrylate

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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title = "Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens",
abstract = "We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.",
author = "Burgio, {V. L.} and A. Martini and M. Avanzini and M. Paulli and R. Rosso",
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journal = "Applied Pathology",
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T1 - Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens

AU - Burgio, V. L.

AU - Martini, A.

AU - Avanzini, M.

AU - Paulli, M.

AU - Rosso, R.

PY - 1984

Y1 - 1984

N2 - We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.

AB - We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.

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