Abstract
We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.
| Original language | English |
|---|---|
| Pages (from-to) | 128-134 |
| Number of pages | 7 |
| Journal | Applied Pathology |
| Volume | 2 |
| Issue number | 3 |
| Publication status | Published - 1984 |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
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Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens. / Burgio, V. L.; Martini, A.; Avanzini, M.; Paulli, M.; Rosso, R.
In: Applied Pathology, Vol. 2, No. 3, 1984, p. 128-134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunofluorescence and enzyme histochemistry on consecutive sections from glycol-methacrylate-embedded bone marrow, lymph node and kidney specimens
AU - Burgio, V. L.
AU - Martini, A.
AU - Avanzini, M.
AU - Paulli, M.
AU - Rosso, R.
PY - 1984
Y1 - 1984
N2 - We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.
AB - We describe an immunohistochemical technique adapted to tissues embedded in glycol-methacrylate (GMA). 1-μm-thick GMA sections from kidney, lymph node and bone marrow biopsies were trypsinized and then incubated with F(ab)2 antisera. GMA sections presented some distinct advantages with respect to compared paraffin and cryostatic sections: reduction of background fluorescence, higher resolution of morphologic details, possibility of studying undecalcified bone marrow specimens and possibility of coupling histochemistry with immunofluorescence on consecutive sections. Moreoever, in a case of lymphoplasmacytoid lymphoma specific immunostaining for membrane IgM was obtained on GMA sections.
UR - http://www.scopus.com/inward/record.url?scp=0021712531&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021712531&partnerID=8YFLogxK
M3 - Article
C2 - 6085791
AN - SCOPUS:0021712531
VL - 2
SP - 128
EP - 134
JO - Applied Pathology
JF - Applied Pathology
SN - 0252-1172
IS - 3
ER -