Immunofluorescence study of patients with neuropathy and IgM M proteins

M. Takatsu, A. P. Hays, N. Latov, G. M. Abrams, R. Nemni, W. H. Sherman, E. Nobile-Orazio, T. Saito, L. Freddo

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Abstract

Immunofluorescence histochemistry was used to study the pathogenesis of polyneuropathy in patients with an IgM M protein. Seventeen patients had an M protein that reacted with myelin-associated glycoprotein (MAG), and their serum immunostained myelin sheaths of normal peripheral nerve of humans and certain other species. The staining was specific for the M protein idiotype and was abolished by prior absorption of serum with MAG. The sural nerve biopsy specimens from these 17 patients had pathological features of primary demyelination and deposits of IgM on the myelin sheaths. Sural nerve specimens of 2 patients with an M protein reactive with chondroitin sulfate showed axonal degeneration and diffuse deposits of IgM in the endoneurium. Serum of one of these patients immunostained connective tissue; the staining was specific for the M protein idiotype and was blocked by absorption of the serum with chondroitin sulfate. The antigenic specificity of the IgM M protein in another 9 patients with neuropathy is not known; however, sural nerve specimens obtained from some of the patients showed axonal degeneration and endoneurial deposits of IgM, and the serum IgM immunostained axons in some instances. The findings suggest that IgM M proteins may cause the neuropathy and that more than one autoantigen is involved.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalAnnals of Neurology
Volume18
Issue number2
DOIs
Publication statusPublished - 1985

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Fluorescent Antibody Technique
Immunoglobulin M
Sural Nerve
Myelin-Associated Glycoprotein
Proteins
Serum
Chondroitin Sulfates
Myelin Sheath
Peripheral Nerves
Staining and Labeling
Polyneuropathies
Autoantigens
Demyelinating Diseases
Connective Tissue
Axons
Epitopes
Biopsy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Takatsu, M., Hays, A. P., Latov, N., Abrams, G. M., Nemni, R., Sherman, W. H., ... Freddo, L. (1985). Immunofluorescence study of patients with neuropathy and IgM M proteins. Annals of Neurology, 18(2), 173-181. https://doi.org/10.1002/ana.410180203

Immunofluorescence study of patients with neuropathy and IgM M proteins. / Takatsu, M.; Hays, A. P.; Latov, N.; Abrams, G. M.; Nemni, R.; Sherman, W. H.; Nobile-Orazio, E.; Saito, T.; Freddo, L.

In: Annals of Neurology, Vol. 18, No. 2, 1985, p. 173-181.

Research output: Contribution to journalArticle

Takatsu, M, Hays, AP, Latov, N, Abrams, GM, Nemni, R, Sherman, WH, Nobile-Orazio, E, Saito, T & Freddo, L 1985, 'Immunofluorescence study of patients with neuropathy and IgM M proteins', Annals of Neurology, vol. 18, no. 2, pp. 173-181. https://doi.org/10.1002/ana.410180203
Takatsu, M. ; Hays, A. P. ; Latov, N. ; Abrams, G. M. ; Nemni, R. ; Sherman, W. H. ; Nobile-Orazio, E. ; Saito, T. ; Freddo, L. / Immunofluorescence study of patients with neuropathy and IgM M proteins. In: Annals of Neurology. 1985 ; Vol. 18, No. 2. pp. 173-181.
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