Immunogenic properties of renal cell carcinoma and the pathogenesis of osteolytic bone metastases

Francesca Perut, Elisabetta Cenni, Ronald E. Unger, C. James Kirkpatrick, Armando Giunti, Nicola Baldini

Research output: Contribution to journalArticle

Abstract

The immunogenic properties of renal cell carcinoma (RCC) on bone osteolysis were investigated. mRNA expression of three proinflammatory cytokines, monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6) and interleukin-8 (IL-8), were determined in a panel of RCC lines (CRBM 1990, ACHN and Caki-1). Moreover proinflammatory cytokine mRNA expression and protein levels of adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and E-selectin, on human umbilical vein endothelial cells (HUVEC) incubated with the conditioned media from RCC lines were evaluated. RCC express mRNA of MCP-1, IL-6 and IL-8 that may induce a proinflammatory phenotype in endothelial cells. mRNA expression of IL-6, and IL-8 was induced on HUVEC treated with the conditioned media from RCC lines and mRNA and protein levels of ICAM-1 and E-selectin were also increased. This study demonstrates the immunogenic properties of renal cell carcinoma, such as pro-inflammatory cytokine secretion and the induction of adhesion molecules (ICAM-1 and E-Sel) by endothelial cells. ICAM-1 binds lymphocyte function-associated antigen-1 (LFA-1), which is expressed by pre-osteoclasts, so that, the observed proinflammatory phenotype in HUVEC may also favour osteoclast recruitment in bone metastases microenvironment. Osteolysis in bone metastases, mediated by this pathway, may be further potentiated by the pro-angiogenic properties of RCC.

Original languageEnglish
Pages (from-to)1387-1393
Number of pages7
JournalInternational Journal of Oncology
Volume34
Issue number5
DOIs
Publication statusPublished - 2009

Keywords

  • Adhesion molecules
  • Endothelial cells
  • Inflammation
  • Osteoclast
  • Renal carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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