Immunoglobulin and T cell receptor β chain gene rearrangement analysis of ocular adnexal lymphoid neoplasms

Clinical and biologic implications

A. Neri, F. A. Jakobiec, P. G. Pelicci, R. Dalla-Favera, D. M. Knowles

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We investigated the organization of the immunoglobulin heavy chain (IgH) and the T cell receptor beta chain (Tβ) gene loci in 20 ocular adnexal and four extraocular lymphoid neoplasms obtained from 18 patients presenting with an ocular adnexal lymphoid neoplasm. Fifteen ocular adnexal and four extraocular lymphoid neoplasms occurring in 13 patients were classified by morphological examination and immunophenotypic analysis as monoclonal B cell lymphomas. Each one of these 19 lymphoid neoplasms exhibited clonal IgH gene rearrangements upon hybridization of EcoRI- or HindIII-digested DNA to a heavy-chain joining region (J(H))-specific DNA probe. The bilateral ocular adnexal monoclonal B cell neoplasms occurring simultaneously in two individuals exhibited identical clonal IgH gene rearrangements, which indicated their derivation from an identical B cell clone. The ocular adnexal and the extraocular monoclonal B cell neoplasms occurring in two of three patients also exhibited identical clonal IgH gene rearrangements, which suggested that they too were derived from an identical B cell clone. Five ocular adnexal lymphoid neoplasms were classified by morphological examination and immunophenotypic analysis as benign, polyclonal pseudolymphomas. Three of these five ocular adnexal lymphoid neoplasm exhibited clonal IgH gene rearrangements, which suggested the presence of monoclonal B cell populations that escaped detection by morphological and immunophenotypic examination. None of the 24 pathological samples exhibited clonal Tβ gene rearrangements upon hybridization of EcoRI- or BamHI-digested DNA to a Tβ gene DNA probe. The results of these studies demonstrate the value of Southern blot hybridization analysis for clonal IgH and Tβ gene rearrangements in the diagnosis, classification, and investigation of extranodal lymphoid neoplasms originating and/or presenting in the ocular adnexa.

Original languageEnglish
Pages (from-to)1519-1529
Number of pages11
JournalBlood
Volume70
Issue number5
Publication statusPublished - 1987

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T-Cell Receptor Genes
Gene Rearrangement
Immunoglobulin Heavy Chains
T-Cell Antigen Receptor
Immunoglobulins
Genes
Immunoglobulin Heavy Chain Genes
Clone cells
B-Lymphocytes
Cells
Neoplasms
DNA Probes
Antigen Receptors, T-Cell, alpha-beta
Clone Cells
T-Cell Receptor beta Genes
DNA
Pseudolymphoma
Joining
B-Cell Lymphoma
Southern Blotting

ASJC Scopus subject areas

  • Hematology

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Immunoglobulin and T cell receptor β chain gene rearrangement analysis of ocular adnexal lymphoid neoplasms : Clinical and biologic implications. / Neri, A.; Jakobiec, F. A.; Pelicci, P. G.; Dalla-Favera, R.; Knowles, D. M.

In: Blood, Vol. 70, No. 5, 1987, p. 1519-1529.

Research output: Contribution to journalArticle

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