Abstract
Background The 'biologically effective dose markers': DNA and protein adducts, are a direct index of carcinogen induced cell damage and an indirect one of genetic susceptibility. This study aimed to examine the dose-response relationship for 4-Aminobiphenyl-DNA adducts in oral cells of smokers and non smokers. Materials and Methods. An immunoperoxidase method with the monoclonal 3C8 antibody, which recognizes 4-Aminobiphenyl-DNA, has been wed for detecting DNA damage in oral cells of 12 smokers and 12 non smokers. Results. Higher staining for 4-Aminobiphenyl-DNA was detected in the cells of smokers (187 ± 42) vs. non smokers (135 ± 35) (p = 0.004), with a twofold range in relative staining for both groups, suggesting individual differences relevance in metabolizing carcinogens and/or repairing DNA damage. Conclusions. This non invasive method requiring small cell amounts is a tool for monitoring large groups of subjects at risk in primary prevention programs.
| Original language | English |
|---|---|
| Pages (from-to) | 2827-2830 |
| Number of pages | 4 |
| Journal | Anticancer Research |
| Volume | 17 |
| Issue number | 4 A |
| Publication status | Published - 1997 |
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Keywords
- 4-aminobiphenyl- DNA adducts
- Oral cells
- Smoke
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Immunohistochemical analysis of 4-Aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers. / Romano, Gianpiero; Mancini, Rita; Fedele, Palma; Curigliano, Giuseppe; Flamini, Giovanna; Giovagnoli, Maria Rosaria; Malara, Natalia; Boninsegna, Alma; Vecchione, Aldo; Santella, Regina M.; Cittadini, Achille.
In: Anticancer Research, Vol. 17, No. 4 A, 1997, p. 2827-2830.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical analysis of 4-Aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers
AU - Romano, Gianpiero
AU - Mancini, Rita
AU - Fedele, Palma
AU - Curigliano, Giuseppe
AU - Flamini, Giovanna
AU - Giovagnoli, Maria Rosaria
AU - Malara, Natalia
AU - Boninsegna, Alma
AU - Vecchione, Aldo
AU - Santella, Regina M.
AU - Cittadini, Achille
PY - 1997
Y1 - 1997
N2 - Background The 'biologically effective dose markers': DNA and protein adducts, are a direct index of carcinogen induced cell damage and an indirect one of genetic susceptibility. This study aimed to examine the dose-response relationship for 4-Aminobiphenyl-DNA adducts in oral cells of smokers and non smokers. Materials and Methods. An immunoperoxidase method with the monoclonal 3C8 antibody, which recognizes 4-Aminobiphenyl-DNA, has been wed for detecting DNA damage in oral cells of 12 smokers and 12 non smokers. Results. Higher staining for 4-Aminobiphenyl-DNA was detected in the cells of smokers (187 ± 42) vs. non smokers (135 ± 35) (p = 0.004), with a twofold range in relative staining for both groups, suggesting individual differences relevance in metabolizing carcinogens and/or repairing DNA damage. Conclusions. This non invasive method requiring small cell amounts is a tool for monitoring large groups of subjects at risk in primary prevention programs.
AB - Background The 'biologically effective dose markers': DNA and protein adducts, are a direct index of carcinogen induced cell damage and an indirect one of genetic susceptibility. This study aimed to examine the dose-response relationship for 4-Aminobiphenyl-DNA adducts in oral cells of smokers and non smokers. Materials and Methods. An immunoperoxidase method with the monoclonal 3C8 antibody, which recognizes 4-Aminobiphenyl-DNA, has been wed for detecting DNA damage in oral cells of 12 smokers and 12 non smokers. Results. Higher staining for 4-Aminobiphenyl-DNA was detected in the cells of smokers (187 ± 42) vs. non smokers (135 ± 35) (p = 0.004), with a twofold range in relative staining for both groups, suggesting individual differences relevance in metabolizing carcinogens and/or repairing DNA damage. Conclusions. This non invasive method requiring small cell amounts is a tool for monitoring large groups of subjects at risk in primary prevention programs.
KW - 4-aminobiphenyl- DNA adducts
KW - Oral cells
KW - Smoke
UR - http://www.scopus.com/inward/record.url?scp=0030857240&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030857240&partnerID=8YFLogxK
M3 - Article
C2 - 9252724
AN - SCOPUS:0030857240
VL - 17
SP - 2827
EP - 2830
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 4 A
ER -