Immunohistochemical analysis of 4-Aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers

Gianpiero Romano, Rita Mancini, Palma Fedele, Giuseppe Curigliano, Giovanna Flamini, Maria Rosaria Giovagnoli, Natalia Malara, Alma Boninsegna, Aldo Vecchione, Regina M. Santella, Achille Cittadini

Research output: Contribution to journalArticle

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Abstract

Background The 'biologically effective dose markers': DNA and protein adducts, are a direct index of carcinogen induced cell damage and an indirect one of genetic susceptibility. This study aimed to examine the dose-response relationship for 4-Aminobiphenyl-DNA adducts in oral cells of smokers and non smokers. Materials and Methods. An immunoperoxidase method with the monoclonal 3C8 antibody, which recognizes 4-Aminobiphenyl-DNA, has been wed for detecting DNA damage in oral cells of 12 smokers and 12 non smokers. Results. Higher staining for 4-Aminobiphenyl-DNA was detected in the cells of smokers (187 ± 42) vs. non smokers (135 ± 35) (p = 0.004), with a twofold range in relative staining for both groups, suggesting individual differences relevance in metabolizing carcinogens and/or repairing DNA damage. Conclusions. This non invasive method requiring small cell amounts is a tool for monitoring large groups of subjects at risk in primary prevention programs.

Original languageEnglish
Pages (from-to)2827-2830
Number of pages4
JournalAnticancer Research
Volume17
Issue number4 A
Publication statusPublished - 1997

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DNA Adducts
Carcinogens
DNA Damage
Staining and Labeling
DNA
Primary Prevention
Genetic Predisposition to Disease
Individuality
Monoclonal Antibodies
4-biphenylamine
Proteins

Keywords

  • 4-aminobiphenyl- DNA adducts
  • Oral cells
  • Smoke

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Romano, G., Mancini, R., Fedele, P., Curigliano, G., Flamini, G., Giovagnoli, M. R., ... Cittadini, A. (1997). Immunohistochemical analysis of 4-Aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers. Anticancer Research, 17(4 A), 2827-2830.

Immunohistochemical analysis of 4-Aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers. / Romano, Gianpiero; Mancini, Rita; Fedele, Palma; Curigliano, Giuseppe; Flamini, Giovanna; Giovagnoli, Maria Rosaria; Malara, Natalia; Boninsegna, Alma; Vecchione, Aldo; Santella, Regina M.; Cittadini, Achille.

In: Anticancer Research, Vol. 17, No. 4 A, 1997, p. 2827-2830.

Research output: Contribution to journalArticle

Romano, G, Mancini, R, Fedele, P, Curigliano, G, Flamini, G, Giovagnoli, MR, Malara, N, Boninsegna, A, Vecchione, A, Santella, RM & Cittadini, A 1997, 'Immunohistochemical analysis of 4-Aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers', Anticancer Research, vol. 17, no. 4 A, pp. 2827-2830.
Romano, Gianpiero ; Mancini, Rita ; Fedele, Palma ; Curigliano, Giuseppe ; Flamini, Giovanna ; Giovagnoli, Maria Rosaria ; Malara, Natalia ; Boninsegna, Alma ; Vecchione, Aldo ; Santella, Regina M. ; Cittadini, Achille. / Immunohistochemical analysis of 4-Aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers. In: Anticancer Research. 1997 ; Vol. 17, No. 4 A. pp. 2827-2830.
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