Immunohistochemical and molecular analysis of BAX, BCL-2 and p53 genes in laryngeal squamous cell carcinomas

Nicola Stefano Fracchiolla, Pasquale Capaccio, Nadia Carboni, Angelo Virgilio Pagliari, Antonino Neri, Domenica Ronchett, Giancarlo Pruner, Maria Grazia Silvotti, Lorenzo Pignataro, Roberto Buffa, Guido Broich

Research output: Contribution to journalArticle

Abstract

Background: We investigated the role of apoptosis and its potential alterations in laryngeal squamous cell carcinomas (LSCCs) by evaluating bax, bcl-2 and p53 protein expression in 50 cases and by characterising the molecular status of the bax and p53 genes. Material and Methods: p53 and bax gene mutations were investigated by means of PCR/SSCP and direct DNA sequencing, and bax, bcl-2 and p53 protein expression by means of immunohistochemistry. Results: We identified p53 gene mutations in 17/50 cases (34%); p53 expression in 26 of the 50 cases (52%); bcl-2 expression in 5/50 cases (10%); bax expression in 32/47 cases (68%). 18/33 cases with a wild type p53 gene overexpressed p53 protein: 12 cases (≃ 66%) were bax+/bcl-2-. Of the remaining cases without p53 protein expression, seven cases (≃ 47%) were bax+/bcl-2-. Conclusions: Our observations suggest that the overexpression of p53 may contribute to the repression of bcl-2 and the induction of bax expression in LSCCs. However, the fact that a number of cases not expressing p53 did not present any clear up-regulation of bax or down-regulation of bcl-2 suggests that bcl-2 and bax may be regulated by various mechanisms other than p53.

Original languageEnglish
Pages (from-to)1043-1051
Number of pages9
JournalAnticancer Research
Volume19
Issue number2 A
Publication statusPublished - 1999

Keywords

  • Bax
  • Bcl-2
  • Larynx
  • P53
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Fracchiolla, N. S., Capaccio, P., Carboni, N., Pagliari, A. V., Neri, A., Ronchett, D., Pruner, G., Silvotti, M. G., Pignataro, L., Buffa, R., & Broich, G. (1999). Immunohistochemical and molecular analysis of BAX, BCL-2 and p53 genes in laryngeal squamous cell carcinomas. Anticancer Research, 19(2 A), 1043-1051.