Immunohistochemical and ultrastructural investigation of neural differentiation in Ewing sarcoma/PNET of bone and soft tissues

A. Franchi, G. Pasquinelli, G. Cenacchi, C. Della Rocca, C. Gambini, M. Bisceglia, G. N. Martinelli, M. Santucci

Research output: Contribution to journalArticle

Abstract

The authors evaluated the role of immunohistochemistry and electron microscopy in defining neural differentiation in 28 cases of Ewing sarcoma/PNET. The panel of primary antibodies used included vimentin, MIC-2, NSE, S-100 protein, leu7, neurofilaments, GFAP, and chromogranin A. Cases were considered undifferentiated when neural markers were absent, poorly differentiated if one neural marker was present, and well differentiated if two or more markers were observed. Cases were also evaluated for the presence of cytoplasmic processes, microtubules, and neurosecretory granules as ultrastructural features of neural differentiation: the tumor was classified as well differentiated if two of these features were present; and poorly differentiated if one was evident; all other cases were considered undifferentiated. According to immunohistochemistry, 10 cases (35.7%) were undifferentiated, 12 cases (42.9%) were poorly differentiated, and 6 (21.4%) were well differentiated. According to the ultrastructural analysis, 10 tumors were undifferentiated (35.7%), 14 poorly differentiated (50%), and 4 well differentiated (14.3%). The overall concordance between the two techniques was low (35.7%), and both modalities were concordant in classifying only 1 well-differentiated, 5 poorly differentiated, and 4 undifferentiated tumors. In conclusion, the authors suggest that investigations devoted to test the prognostic significance of neural differentiation in these neoplasms should employ both immunohistochemistry and electron microscopy, separately and in combination, to assess what is the most effective choice for predicting the clinical course.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalUltrastructural Pathology
Volume25
Issue number3
Publication statusPublished - 2001

Fingerprint

Primitive Neuroectodermal Tumors
Ewing's Sarcoma
markers
bones
Tumors
Bone
tumors
cancer
Tissue
Bone and Bones
Electron microscopy
Immunohistochemistry
electron microscopy
microscopy
neoplasms
Neoplasms
Electron Microscopy
classifying
antibodies
Antibodies

Keywords

  • Electron microscopy
  • Ewing sarcoma
  • Immunohistochemistry
  • Neural differentiation
  • Peripheral neuroectodermal tumor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Instrumentation
  • Structural Biology

Cite this

Franchi, A., Pasquinelli, G., Cenacchi, G., Della Rocca, C., Gambini, C., Bisceglia, M., ... Santucci, M. (2001). Immunohistochemical and ultrastructural investigation of neural differentiation in Ewing sarcoma/PNET of bone and soft tissues. Ultrastructural Pathology, 25(3), 219-225.

Immunohistochemical and ultrastructural investigation of neural differentiation in Ewing sarcoma/PNET of bone and soft tissues. / Franchi, A.; Pasquinelli, G.; Cenacchi, G.; Della Rocca, C.; Gambini, C.; Bisceglia, M.; Martinelli, G. N.; Santucci, M.

In: Ultrastructural Pathology, Vol. 25, No. 3, 2001, p. 219-225.

Research output: Contribution to journalArticle

Franchi, A, Pasquinelli, G, Cenacchi, G, Della Rocca, C, Gambini, C, Bisceglia, M, Martinelli, GN & Santucci, M 2001, 'Immunohistochemical and ultrastructural investigation of neural differentiation in Ewing sarcoma/PNET of bone and soft tissues', Ultrastructural Pathology, vol. 25, no. 3, pp. 219-225.
Franchi A, Pasquinelli G, Cenacchi G, Della Rocca C, Gambini C, Bisceglia M et al. Immunohistochemical and ultrastructural investigation of neural differentiation in Ewing sarcoma/PNET of bone and soft tissues. Ultrastructural Pathology. 2001;25(3):219-225.
Franchi, A. ; Pasquinelli, G. ; Cenacchi, G. ; Della Rocca, C. ; Gambini, C. ; Bisceglia, M. ; Martinelli, G. N. ; Santucci, M. / Immunohistochemical and ultrastructural investigation of neural differentiation in Ewing sarcoma/PNET of bone and soft tissues. In: Ultrastructural Pathology. 2001 ; Vol. 25, No. 3. pp. 219-225.
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abstract = "The authors evaluated the role of immunohistochemistry and electron microscopy in defining neural differentiation in 28 cases of Ewing sarcoma/PNET. The panel of primary antibodies used included vimentin, MIC-2, NSE, S-100 protein, leu7, neurofilaments, GFAP, and chromogranin A. Cases were considered undifferentiated when neural markers were absent, poorly differentiated if one neural marker was present, and well differentiated if two or more markers were observed. Cases were also evaluated for the presence of cytoplasmic processes, microtubules, and neurosecretory granules as ultrastructural features of neural differentiation: the tumor was classified as well differentiated if two of these features were present; and poorly differentiated if one was evident; all other cases were considered undifferentiated. According to immunohistochemistry, 10 cases (35.7{\%}) were undifferentiated, 12 cases (42.9{\%}) were poorly differentiated, and 6 (21.4{\%}) were well differentiated. According to the ultrastructural analysis, 10 tumors were undifferentiated (35.7{\%}), 14 poorly differentiated (50{\%}), and 4 well differentiated (14.3{\%}). The overall concordance between the two techniques was low (35.7{\%}), and both modalities were concordant in classifying only 1 well-differentiated, 5 poorly differentiated, and 4 undifferentiated tumors. In conclusion, the authors suggest that investigations devoted to test the prognostic significance of neural differentiation in these neoplasms should employ both immunohistochemistry and electron microscopy, separately and in combination, to assess what is the most effective choice for predicting the clinical course.",
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AU - Martinelli, G. N.

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