Immunohistochemical characterization of coronary thrombi in allograft vascular disease

Eloisa Arbustini, Barbara Dal Bello, Patrizia Morbini, Antonello Gavazzi, Giuseppe Specchia, Mario Viganò

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background. Coronary thrombosis is a frequent complication of allograft vascular disease (AVD) in cardiac transplant recipients. No data are available on thrombus composition in these hearts. Methods. The present study aimed at characterizing thrombus components in coronary arteries from transplanted hearts with AVD, using single and double immunostain with anti- gpIIb-IIIa, anti-fibrin, and anti-endothelial antibodies. The pathologic series consists of 55 grafts survived longer than 2 months, and obtained from 55 patients deceased (n=44) or undergone repeat transplantation (n=11). Results. Mural thrombi were found in multiple segments of 75 of 440 total coronary vessels (17%) (recent in 33, organizing in 28, and organized in 14), whereas occlusive thrombi were found in 19 vessels (8 recent and 11 with multichannel pattern of organization). Recent and thin mural thrombi were mostly constituted of CD41a- and CD61-positive platelets; the amount of fibrin progressively increased with the increase of thrombus size. In organizing mural thrombi, gpIIb-IIIa immunostain was still present. Fibrin was the only identifiable thrombus component in old mural thrombi embedded within the intimal lesions. Recent occlusive thrombi immunoreacted both with antiCD41a and anti-CD61 and with anti-fibrin antibodies, whereas organized occlusive thrombi with multichannel pattern exclusively immunoreacted with anti-fibrin antibodies. Double immunostain showed that mural thrombi were stratified on de-endothelized arterial segments. Conclusions. Thrombus composition is related to both type and 'age' of thrombus, with platelets as the early and major components of mural microthrombi at one end of the spectrum, and fibrin as the dominant component of occlusive thrombi at the other end.

Original languageEnglish
Pages (from-to)1095-1101
Number of pages7
JournalTransplantation
Volume69
Issue number6
Publication statusPublished - Mar 27 2000

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Vascular Diseases
Allografts
Thrombosis
Fibrin
Anti-Idiotypic Antibodies
Coronary Vessels
Blood Platelets
Tunica Intima
Coronary Thrombosis
Transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Arbustini, E., Bello, B. D., Morbini, P., Gavazzi, A., Specchia, G., & Viganò, M. (2000). Immunohistochemical characterization of coronary thrombi in allograft vascular disease. Transplantation, 69(6), 1095-1101.

Immunohistochemical characterization of coronary thrombi in allograft vascular disease. / Arbustini, Eloisa; Bello, Barbara Dal; Morbini, Patrizia; Gavazzi, Antonello; Specchia, Giuseppe; Viganò, Mario.

In: Transplantation, Vol. 69, No. 6, 27.03.2000, p. 1095-1101.

Research output: Contribution to journalArticle

Arbustini, E, Bello, BD, Morbini, P, Gavazzi, A, Specchia, G & Viganò, M 2000, 'Immunohistochemical characterization of coronary thrombi in allograft vascular disease', Transplantation, vol. 69, no. 6, pp. 1095-1101.
Arbustini, Eloisa ; Bello, Barbara Dal ; Morbini, Patrizia ; Gavazzi, Antonello ; Specchia, Giuseppe ; Viganò, Mario. / Immunohistochemical characterization of coronary thrombi in allograft vascular disease. In: Transplantation. 2000 ; Vol. 69, No. 6. pp. 1095-1101.
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abstract = "Background. Coronary thrombosis is a frequent complication of allograft vascular disease (AVD) in cardiac transplant recipients. No data are available on thrombus composition in these hearts. Methods. The present study aimed at characterizing thrombus components in coronary arteries from transplanted hearts with AVD, using single and double immunostain with anti- gpIIb-IIIa, anti-fibrin, and anti-endothelial antibodies. The pathologic series consists of 55 grafts survived longer than 2 months, and obtained from 55 patients deceased (n=44) or undergone repeat transplantation (n=11). Results. Mural thrombi were found in multiple segments of 75 of 440 total coronary vessels (17{\%}) (recent in 33, organizing in 28, and organized in 14), whereas occlusive thrombi were found in 19 vessels (8 recent and 11 with multichannel pattern of organization). Recent and thin mural thrombi were mostly constituted of CD41a- and CD61-positive platelets; the amount of fibrin progressively increased with the increase of thrombus size. In organizing mural thrombi, gpIIb-IIIa immunostain was still present. Fibrin was the only identifiable thrombus component in old mural thrombi embedded within the intimal lesions. Recent occlusive thrombi immunoreacted both with antiCD41a and anti-CD61 and with anti-fibrin antibodies, whereas organized occlusive thrombi with multichannel pattern exclusively immunoreacted with anti-fibrin antibodies. Double immunostain showed that mural thrombi were stratified on de-endothelized arterial segments. Conclusions. Thrombus composition is related to both type and 'age' of thrombus, with platelets as the early and major components of mural microthrombi at one end of the spectrum, and fibrin as the dominant component of occlusive thrombi at the other end.",
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AU - Bello, Barbara Dal

AU - Morbini, Patrizia

AU - Gavazzi, Antonello

AU - Specchia, Giuseppe

AU - Viganò, Mario

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N2 - Background. Coronary thrombosis is a frequent complication of allograft vascular disease (AVD) in cardiac transplant recipients. No data are available on thrombus composition in these hearts. Methods. The present study aimed at characterizing thrombus components in coronary arteries from transplanted hearts with AVD, using single and double immunostain with anti- gpIIb-IIIa, anti-fibrin, and anti-endothelial antibodies. The pathologic series consists of 55 grafts survived longer than 2 months, and obtained from 55 patients deceased (n=44) or undergone repeat transplantation (n=11). Results. Mural thrombi were found in multiple segments of 75 of 440 total coronary vessels (17%) (recent in 33, organizing in 28, and organized in 14), whereas occlusive thrombi were found in 19 vessels (8 recent and 11 with multichannel pattern of organization). Recent and thin mural thrombi were mostly constituted of CD41a- and CD61-positive platelets; the amount of fibrin progressively increased with the increase of thrombus size. In organizing mural thrombi, gpIIb-IIIa immunostain was still present. Fibrin was the only identifiable thrombus component in old mural thrombi embedded within the intimal lesions. Recent occlusive thrombi immunoreacted both with antiCD41a and anti-CD61 and with anti-fibrin antibodies, whereas organized occlusive thrombi with multichannel pattern exclusively immunoreacted with anti-fibrin antibodies. Double immunostain showed that mural thrombi were stratified on de-endothelized arterial segments. Conclusions. Thrombus composition is related to both type and 'age' of thrombus, with platelets as the early and major components of mural microthrombi at one end of the spectrum, and fibrin as the dominant component of occlusive thrombi at the other end.

AB - Background. Coronary thrombosis is a frequent complication of allograft vascular disease (AVD) in cardiac transplant recipients. No data are available on thrombus composition in these hearts. Methods. The present study aimed at characterizing thrombus components in coronary arteries from transplanted hearts with AVD, using single and double immunostain with anti- gpIIb-IIIa, anti-fibrin, and anti-endothelial antibodies. The pathologic series consists of 55 grafts survived longer than 2 months, and obtained from 55 patients deceased (n=44) or undergone repeat transplantation (n=11). Results. Mural thrombi were found in multiple segments of 75 of 440 total coronary vessels (17%) (recent in 33, organizing in 28, and organized in 14), whereas occlusive thrombi were found in 19 vessels (8 recent and 11 with multichannel pattern of organization). Recent and thin mural thrombi were mostly constituted of CD41a- and CD61-positive platelets; the amount of fibrin progressively increased with the increase of thrombus size. In organizing mural thrombi, gpIIb-IIIa immunostain was still present. Fibrin was the only identifiable thrombus component in old mural thrombi embedded within the intimal lesions. Recent occlusive thrombi immunoreacted both with antiCD41a and anti-CD61 and with anti-fibrin antibodies, whereas organized occlusive thrombi with multichannel pattern exclusively immunoreacted with anti-fibrin antibodies. Double immunostain showed that mural thrombi were stratified on de-endothelized arterial segments. Conclusions. Thrombus composition is related to both type and 'age' of thrombus, with platelets as the early and major components of mural microthrombi at one end of the spectrum, and fibrin as the dominant component of occlusive thrombi at the other end.

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