Immunohistochemical detection of the multidrug transport protein P170 in human normal tissues and malignant lymphomas

S. A. Pileri, E. Sabattini, B. Falini, P. L. Tazzari, F. Gherlinzoni, M. G. Michieli, D. Damiani, L. Zucchini, M. Gobbi, T. Tsuruo, M. Baccarani

Research output: Contribution to journalArticle

Abstract

Two monoclonal antibodies, MRK16 and C219, both directed at the 170 kDa P-glycoprotein multidrug resistance agent, were applied to frozen sections or cytospin preparations from normal human tissues and 60 non-Hodgkin's malignant lymphomas. Adrenal gland, kidney, liver and pancreas were always stained by the reagents, albeit with slightly different patterns. Brain capillaries as well as macrophages and some elements of the bone marrow, peripheral blood, ovarian stroma and colonic, gastric and jejunal mucosa were positive in all examined preparations. There were differences in the staining patterns with the two antibodies. Among the 60 non-Hodgkin's lymphomas, 25 contained a number of positive cells, which ranged from 2% to 100%. No correlation was seen between the expression of P170 and histological type, stage, clinical symptoms or growth fraction. A close relationship was shown between the presence of P170 positive elements and the clinical course of the disease (P <0.001 ).

Original languageEnglish
Pages (from-to)131-140
Number of pages10
JournalHistopathology
Volume19
Issue number2
Publication statusPublished - 1991

Keywords

  • malignant lymphoma
  • multidrug resistance
  • normal tissues
  • P-glycoprotein 170

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

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    Pileri, S. A., Sabattini, E., Falini, B., Tazzari, P. L., Gherlinzoni, F., Michieli, M. G., Damiani, D., Zucchini, L., Gobbi, M., Tsuruo, T., & Baccarani, M. (1991). Immunohistochemical detection of the multidrug transport protein P170 in human normal tissues and malignant lymphomas. Histopathology, 19(2), 131-140.