Immunohistochemical evidence of vanilloid receptor 1 in normal human urinary bladder

Massimo Lazzeri, Maria Giuliana Vannucchi, Claudio Zardo, Michele Spinelli, Patrizia Beneforti, Damiano Turini, Maria Simonetta Faussone-Pellegrini

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Abstract

Experimental and clinical evidences have shown the importance of the vanilloid receptor 1 (TRPV1) in the lower urinary tract. In humans, this receptor has been detected in nerve endings of primary sensory neurons, smooth muscle and connective tissue cells and in the rat also in the urothelium. The aim of this study is to identify, by immunohistochemistry, the cell types expressing TRPV1 in the human urinary bladder. Specimens, obtained from normal urinary bladder by multiple biopsy and from ureter at the time of radical nefrectomy for renal cell carcinoma, were fixed and frozen. Full-thickness sections were processed for light and fluorescence microscopes. To label the TRPV1, three polyclonal antibodies were used: the anti-capsaicin receptor, the anti-VR1 (N-15) and the anti-VR1 (C-15). Urothelium, smooth muscle cells, mast cells and endothelium were labelled and the labelling was intracytoplasmatic. In the urothelial cells, the labelling was slightly granular. In the bladder urothelium, the superficial cells were more intensely stained than the basal and club-shaped cells. VR1-positive nerve fibers were seen running single and/or in groups in the sub-urothelium and as single varicose fibers in the muscle coat, and VR1-positive nerve endings in the urothelium. The present findings provide the evidence of the presence of TRPV1 on normal human urothelium where it could have important implications in the mechanism of action of intravesical vanilloids (capsaicin and resiniferatoxin).

Original languageEnglish
Pages (from-to)792-798
Number of pages7
JournalEuropean Urology
Volume46
Issue number6
DOIs
Publication statusPublished - Dec 2004

Fingerprint

Urothelium
Urinary Bladder
Nerve Endings
Connective Tissue Cells
TRPV Cation Channels
Muscles
Capsaicin
Sensory Receptor Cells
Ureter
Urinary Tract
Nerve Fibers
Renal Cell Carcinoma
Mast Cells
Running
Smooth Muscle Myocytes
Endothelium
Smooth Muscle
vanilloid receptor subtype 1
Fluorescence
Immunohistochemistry

Keywords

  • Bladder
  • Immunohistochemistry
  • Sensory nerves
  • Urothelium
  • Vanilloid receptor

ASJC Scopus subject areas

  • Urology

Cite this

Lazzeri, M., Vannucchi, M. G., Zardo, C., Spinelli, M., Beneforti, P., Turini, D., & Faussone-Pellegrini, M. S. (2004). Immunohistochemical evidence of vanilloid receptor 1 in normal human urinary bladder. European Urology, 46(6), 792-798. https://doi.org/10.1016/j.eururo.2004.08.007

Immunohistochemical evidence of vanilloid receptor 1 in normal human urinary bladder. / Lazzeri, Massimo; Vannucchi, Maria Giuliana; Zardo, Claudio; Spinelli, Michele; Beneforti, Patrizia; Turini, Damiano; Faussone-Pellegrini, Maria Simonetta.

In: European Urology, Vol. 46, No. 6, 12.2004, p. 792-798.

Research output: Contribution to journalArticle

Lazzeri, M, Vannucchi, MG, Zardo, C, Spinelli, M, Beneforti, P, Turini, D & Faussone-Pellegrini, MS 2004, 'Immunohistochemical evidence of vanilloid receptor 1 in normal human urinary bladder', European Urology, vol. 46, no. 6, pp. 792-798. https://doi.org/10.1016/j.eururo.2004.08.007
Lazzeri, Massimo ; Vannucchi, Maria Giuliana ; Zardo, Claudio ; Spinelli, Michele ; Beneforti, Patrizia ; Turini, Damiano ; Faussone-Pellegrini, Maria Simonetta. / Immunohistochemical evidence of vanilloid receptor 1 in normal human urinary bladder. In: European Urology. 2004 ; Vol. 46, No. 6. pp. 792-798.
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