Immunohistochemical expression of type VI collagen in superficial fibromatoses

G. Magro, A. Colombatti, S. Lanzafame

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The expression of type VI collagen was studied immunohistochemically in 26 cases of superficial fibromatoses (palmar, plantar and penile) using an immunoperoxidase method for light microscopic visualization. The polyclonal antibody against type VI collagen used in this study was isolated from human placenta and its specifity was tested by immunoblotting assay. All cases consisted of multiple nodules showing a variable degree of cellularity and fibrosis. Depending on the predominant histological appearance of these nodules, each case was assigned to the three following phases: proliferative, involutional and residual. Morphologically normal palmar and plantar aponeuroses were included as controls. Immunohistochemical findings showed that type VI collagen was present as longitudinal thin fibers in normal palmar and plantar aponeuroses. A differential expression of this collagen was found in the different stages of superficial fibromatoses. Type VI collagen was markedly expressed as a distinct fibrillar network in the extracellular matrix (ECM) surrounding proliferating stromal cells in proliferative and involutional phases. Its expression completely disappeared from the connective tissue undergoing fibrotic transformation during involutional and residual phases. The results of the present study suggest that type VI collagen is an extracellular marker of stromal tissue proliferation and is involved in the early phases of tissue remodelling occurring in the superficial fibromatoses.

Original languageEnglish
Pages (from-to)1023-1028
Number of pages6
JournalPathology Research and Practice
Volume191
Issue number10
Publication statusPublished - 1995

Fingerprint

Collagen Type VI
Fibroma
Penile Induration
Dupuytren Contracture
Stromal Cells
Immunoblotting
Connective Tissue
Placenta
Extracellular Matrix
Fibrosis
Collagen
Light
Antibodies

Keywords

  • Fibromatoses
  • Immunohistochemistry
  • Type VI collagen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Magro, G., Colombatti, A., & Lanzafame, S. (1995). Immunohistochemical expression of type VI collagen in superficial fibromatoses. Pathology Research and Practice, 191(10), 1023-1028.

Immunohistochemical expression of type VI collagen in superficial fibromatoses. / Magro, G.; Colombatti, A.; Lanzafame, S.

In: Pathology Research and Practice, Vol. 191, No. 10, 1995, p. 1023-1028.

Research output: Contribution to journalArticle

Magro, G, Colombatti, A & Lanzafame, S 1995, 'Immunohistochemical expression of type VI collagen in superficial fibromatoses', Pathology Research and Practice, vol. 191, no. 10, pp. 1023-1028.
Magro, G. ; Colombatti, A. ; Lanzafame, S. / Immunohistochemical expression of type VI collagen in superficial fibromatoses. In: Pathology Research and Practice. 1995 ; Vol. 191, No. 10. pp. 1023-1028.
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