Immunohistochemical localization of human immunoglobulins and lysozyme in epoxy-embedded lymph nodes. Effect of different fixatives and of proteolytic digestion

P. Dell'Orto, G. Viale, R. Colombi, P. Braidotti, G. Coggi

Research output: Contribution to journalArticlepeer-review

Abstract

The postembedding immunoperoxidase staining technique for the localization of immunoglobulins (light and heavy chains) and of lysozyme has been successfully applied to epoxy-embedded human lymph nodes, after removal of the resin. Glutaraldehyde-containing fixatives appear to be suitable for the immunohistochemical localization of human immunoglobulins and lysozyme, provided that the masked antigenicity of these proteins is recovered by proteolytic digestion of the tissue sections using 0.4% pepsin or 0.1% trypsin. Nonglutaraldehyde-containing fixatives allow the immunolocalization of human immunoglobulins without any enzymatic pretreatment. This study shows that tissues routinely fixed in glutaraldehyde and embedded for ultrastructural investigations are actually suitable for immunohistochemical studies on human immunoglobulins and lysozyme.

Original languageEnglish
Pages (from-to)630-636
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume30
Issue number7
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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