Immunohistochemical Localization of Peroxisomal Enzymes during Rat Embryonic Development

Roberta Nardacci, Ilaria Falciatori, Sandra Moreno, Stefania Stefanini

Research output: Contribution to journalArticle

Abstract

Peroxisomes are cytoplasmic organelles involved in a variety of metabolic pathways. Thus far, the morphological and biochemical features of peroxisomes have been extensively characterized in adult tissues. However, the existence of congenital peroxisomal disorders, primarily affecting tissue differentiation, emphasizes the importance of these organelles in the early stages of organogenesis. We investigated the occurrence and tissue distribution of three peroxisomal enzymes in rat embryos at various developmental stages. By means of a highly sensitive biotinyl-tyramide protocol, catalase, acyl-CoA oxidase, and ketoacyl-CoA thiolase were detected in embryonic tissues where peroxisomes had not thus far been recognized, i.e., adrenal and pancreatic parenchyma, choroid plexus, neuroblasts of cranial and spinal ganglia and myenteric plexus, and chondroblasts of certain skeletal structures. In other tissues, i.e., gut epithelium and neuroblasts of some CNS areas, they were identified earlier than previously. In select CNS areas, ultrastructural catalase cytochemistry allowed identification of actively proliferating organelles at early developmental stages in several cell types. Our data show that in most organs maturation of peroxisomes parallels the acquirement of specific functions, mainly related to lipid metabolism, thus supporting an involvement of the organelles in tissue differentiation.

Original languageEnglish
Pages (from-to)423-436
Number of pages14
JournalJournal of Histochemistry and Cytochemistry
Volume52
Issue number4
Publication statusPublished - Apr 2004

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Peroxisomes
Embryonic Development
Organelles
Enzymes
Catalase
Peroxisomal Disorders
Acyl-CoA Oxidase
Myenteric Plexus
Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Histocytochemistry
Choroid Plexus
Organogenesis
Spinal Ganglia
Tissue Distribution
Coenzyme A
Chondrocytes
Metabolic Networks and Pathways
Lipid Metabolism
Embryonic Structures
Epithelium

Keywords

  • Biogenesis
  • Brain
  • Catalase
  • Fatty acid β-oxidation
  • Ketoacyl-CoA thiolase
  • Kidney
  • Liver

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Immunohistochemical Localization of Peroxisomal Enzymes during Rat Embryonic Development. / Nardacci, Roberta; Falciatori, Ilaria; Moreno, Sandra; Stefanini, Stefania.

In: Journal of Histochemistry and Cytochemistry, Vol. 52, No. 4, 04.2004, p. 423-436.

Research output: Contribution to journalArticle

Nardacci, Roberta ; Falciatori, Ilaria ; Moreno, Sandra ; Stefanini, Stefania. / Immunohistochemical Localization of Peroxisomal Enzymes during Rat Embryonic Development. In: Journal of Histochemistry and Cytochemistry. 2004 ; Vol. 52, No. 4. pp. 423-436.
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