Immunohistochemical mismatch repair proteins expression in colorectal cancer

Anna Maria Valentini; Raffaele Armentano; Michele Pirrelli; Mattia Gentile; Maria Lucia Caruso

doi:10.1097/01.pai.0000155193.92167.6a

Immunohistochemical mismatch repair proteins expression in colorectal cancer

Anna Maria Valentini, Raffaele Armentano, Michele Pirrelli, Mattia Gentile, Maria Lucia Caruso

Ente Ospedaliero Specializzato in Gastroenterologia "Saverio de Bellis"

Research output: Contribution to journal › Article › peer-review

Abstract

Immunohistochemical (IHC) assessment of the mismatch repair proteins has been proposed as an alternative strategy to molecular biology for evaluating the unstable phenotype of tumors. With the aim of introducing IHC analysis as a routine diagnostic test, the authors compared the expression of MLH1 and MSH2 proteins with a PCR-based microsatellite assay. The concordance rate between the two methods was 90% after IHC evaluation of two different areas of each tumor. These results show that IHC may be as efficient as PCR in detecting unstable phenotype by using only one surgical or biopsy sample.

Original language	English
Pages (from-to)	42-45
Number of pages	4
Journal	Applied Immunohistochemistry and Molecular Morphology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1097/01.pai.0000155193.92167.6a
Publication status	Published - Mar 2006

Keywords

Mismatch repair protein
MLH1
MSH2

ASJC Scopus subject areas

Anatomy
Medical Laboratory Technology

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10.1097/01.pai.0000155193.92167.6a

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title = "Immunohistochemical mismatch repair proteins expression in colorectal cancer",

abstract = "Immunohistochemical (IHC) assessment of the mismatch repair proteins has been proposed as an alternative strategy to molecular biology for evaluating the unstable phenotype of tumors. With the aim of introducing IHC analysis as a routine diagnostic test, the authors compared the expression of MLH1 and MSH2 proteins with a PCR-based microsatellite assay. The concordance rate between the two methods was 90% after IHC evaluation of two different areas of each tumor. These results show that IHC may be as efficient as PCR in detecting unstable phenotype by using only one surgical or biopsy sample.",

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AU - Caruso, Maria Lucia

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AB - Immunohistochemical (IHC) assessment of the mismatch repair proteins has been proposed as an alternative strategy to molecular biology for evaluating the unstable phenotype of tumors. With the aim of introducing IHC analysis as a routine diagnostic test, the authors compared the expression of MLH1 and MSH2 proteins with a PCR-based microsatellite assay. The concordance rate between the two methods was 90% after IHC evaluation of two different areas of each tumor. These results show that IHC may be as efficient as PCR in detecting unstable phenotype by using only one surgical or biopsy sample.

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KW - MSH2

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Immunohistochemical mismatch repair proteins expression in colorectal cancer

Abstract

Keywords

ASJC Scopus subject areas

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