Immunohistochemical study of six cases of Taylor's type focal cortical dysplasia: Correlation with electroclinical data

Dominique Lurton, Elza Marcia Yacubian, Emilio Garrido Sanabria, Alexandre Valotta Da Silva, Regina Vianna, Eliana Garzon, Americo Sakamoto, Roberto Spreafico, Esper Abrão Cavalheiro

Research output: Contribution to journalArticle

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Abstract

Purpose: Cortical specimens from six patients operated on for drug-resistant epilepsy diagnosed as Taylor's type focal cortical dysplasia were submitted to neuropathological and immunohistochemical studies. Methods: All patients were submitted to presurgical investigations including clinical and neuropsychological evaluations, EEG/video telemetry of ictal and interictal events, magnetic resonance imaging, and ictal and interictal single-photon emission computed tomography (SPECT). Recordings from electrocorticography (ECoG) were obtained in four cases and from subdural electrode implantation in two. Postsurgical follow-up was assessed according to Engel's score. Immunohistochemistry (IHC) was processed for parvalbumin (PV), calbindin D28-K (CB), nonphosphorylated neurofilaments (SMI-311), glial fibrillary acidic protein (GFAP) in all cases. Results: We found continuous/quasi-continuous spikes and sharp-wave patterns in three cases and frequent repetitive bursting of polyspikes and ECoG seizures in two cases. Every patient showed cortical dyslamination, abnormal and giant neurons, and balloon cells. GFAP immunoreactivity was found in astrocytes and some balloon cells that were less intensely stained. Nonphosphorylated neurofilaments SMI-311 immunoreactivity was found in normal and giant neurons and in some balloon cells, making visible thin neuropils. PV immunoreactivity was present in normal interneurons and in fibers in layers IV-V. PV-negative balloon cells were surrounded by abundant PV-positive fibers. CB immunoreactivity was found mostly in interneurons in layers II-III. Conclusions: Our research is inconclusive. More cases should be investigated, and we must draw more accurate anatomic correlations between the ECoG recordings and surgical specimens studied with IHC.

Original languageEnglish
Pages (from-to)217-219
Number of pages3
JournalEpilepsia
Volume43
Issue numberSUPPL. 5
DOIs
Publication statusPublished - 2002

Fingerprint

Parvalbumins
Intermediate Filaments
Glial Fibrillary Acidic Protein
Interneurons
Immunohistochemistry
Stroke
Neurons
Calbindins
Telemetry
Neuropil
Single-Photon Emission-Computed Tomography
Astrocytes
Electroencephalography
Electrodes
Seizures
Magnetic Resonance Imaging
Focal cortical dysplasia of Taylor
Research
Electrocorticography

Keywords

  • Cortical dysplasia
  • Electrocorticography
  • Immunohistochemistry
  • Taylor

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Lurton, D., Yacubian, E. M., Sanabria, E. G., Da Silva, A. V., Vianna, R., Garzon, E., ... Cavalheiro, E. A. (2002). Immunohistochemical study of six cases of Taylor's type focal cortical dysplasia: Correlation with electroclinical data. Epilepsia, 43(SUPPL. 5), 217-219. https://doi.org/10.1046/j.1528-1157.43.s.5.42.x

Immunohistochemical study of six cases of Taylor's type focal cortical dysplasia : Correlation with electroclinical data. / Lurton, Dominique; Yacubian, Elza Marcia; Sanabria, Emilio Garrido; Da Silva, Alexandre Valotta; Vianna, Regina; Garzon, Eliana; Sakamoto, Americo; Spreafico, Roberto; Cavalheiro, Esper Abrão.

In: Epilepsia, Vol. 43, No. SUPPL. 5, 2002, p. 217-219.

Research output: Contribution to journalArticle

Lurton, D, Yacubian, EM, Sanabria, EG, Da Silva, AV, Vianna, R, Garzon, E, Sakamoto, A, Spreafico, R & Cavalheiro, EA 2002, 'Immunohistochemical study of six cases of Taylor's type focal cortical dysplasia: Correlation with electroclinical data', Epilepsia, vol. 43, no. SUPPL. 5, pp. 217-219. https://doi.org/10.1046/j.1528-1157.43.s.5.42.x
Lurton, Dominique ; Yacubian, Elza Marcia ; Sanabria, Emilio Garrido ; Da Silva, Alexandre Valotta ; Vianna, Regina ; Garzon, Eliana ; Sakamoto, Americo ; Spreafico, Roberto ; Cavalheiro, Esper Abrão. / Immunohistochemical study of six cases of Taylor's type focal cortical dysplasia : Correlation with electroclinical data. In: Epilepsia. 2002 ; Vol. 43, No. SUPPL. 5. pp. 217-219.
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T1 - Immunohistochemical study of six cases of Taylor's type focal cortical dysplasia

T2 - Correlation with electroclinical data

AU - Lurton, Dominique

AU - Yacubian, Elza Marcia

AU - Sanabria, Emilio Garrido

AU - Da Silva, Alexandre Valotta

AU - Vianna, Regina

AU - Garzon, Eliana

AU - Sakamoto, Americo

AU - Spreafico, Roberto

AU - Cavalheiro, Esper Abrão

PY - 2002

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N2 - Purpose: Cortical specimens from six patients operated on for drug-resistant epilepsy diagnosed as Taylor's type focal cortical dysplasia were submitted to neuropathological and immunohistochemical studies. Methods: All patients were submitted to presurgical investigations including clinical and neuropsychological evaluations, EEG/video telemetry of ictal and interictal events, magnetic resonance imaging, and ictal and interictal single-photon emission computed tomography (SPECT). Recordings from electrocorticography (ECoG) were obtained in four cases and from subdural electrode implantation in two. Postsurgical follow-up was assessed according to Engel's score. Immunohistochemistry (IHC) was processed for parvalbumin (PV), calbindin D28-K (CB), nonphosphorylated neurofilaments (SMI-311), glial fibrillary acidic protein (GFAP) in all cases. Results: We found continuous/quasi-continuous spikes and sharp-wave patterns in three cases and frequent repetitive bursting of polyspikes and ECoG seizures in two cases. Every patient showed cortical dyslamination, abnormal and giant neurons, and balloon cells. GFAP immunoreactivity was found in astrocytes and some balloon cells that were less intensely stained. Nonphosphorylated neurofilaments SMI-311 immunoreactivity was found in normal and giant neurons and in some balloon cells, making visible thin neuropils. PV immunoreactivity was present in normal interneurons and in fibers in layers IV-V. PV-negative balloon cells were surrounded by abundant PV-positive fibers. CB immunoreactivity was found mostly in interneurons in layers II-III. Conclusions: Our research is inconclusive. More cases should be investigated, and we must draw more accurate anatomic correlations between the ECoG recordings and surgical specimens studied with IHC.

AB - Purpose: Cortical specimens from six patients operated on for drug-resistant epilepsy diagnosed as Taylor's type focal cortical dysplasia were submitted to neuropathological and immunohistochemical studies. Methods: All patients were submitted to presurgical investigations including clinical and neuropsychological evaluations, EEG/video telemetry of ictal and interictal events, magnetic resonance imaging, and ictal and interictal single-photon emission computed tomography (SPECT). Recordings from electrocorticography (ECoG) were obtained in four cases and from subdural electrode implantation in two. Postsurgical follow-up was assessed according to Engel's score. Immunohistochemistry (IHC) was processed for parvalbumin (PV), calbindin D28-K (CB), nonphosphorylated neurofilaments (SMI-311), glial fibrillary acidic protein (GFAP) in all cases. Results: We found continuous/quasi-continuous spikes and sharp-wave patterns in three cases and frequent repetitive bursting of polyspikes and ECoG seizures in two cases. Every patient showed cortical dyslamination, abnormal and giant neurons, and balloon cells. GFAP immunoreactivity was found in astrocytes and some balloon cells that were less intensely stained. Nonphosphorylated neurofilaments SMI-311 immunoreactivity was found in normal and giant neurons and in some balloon cells, making visible thin neuropils. PV immunoreactivity was present in normal interneurons and in fibers in layers IV-V. PV-negative balloon cells were surrounded by abundant PV-positive fibers. CB immunoreactivity was found mostly in interneurons in layers II-III. Conclusions: Our research is inconclusive. More cases should be investigated, and we must draw more accurate anatomic correlations between the ECoG recordings and surgical specimens studied with IHC.

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