Immunohistochemical study of subepidermal connective of molluscan integument

S. Corbetta, A. Bairati, L. Vitellaro Zuccarello

Research output: Contribution to journalArticle

Abstract

Sections of integument from gastropod, bivalve and cephalopod species were studied immunohistochemically to determine reactivity to antibody against the type I-like collagen from Sepia cartilage and antibodies against components of the extracellular matrix (ECM) of vertebrate connective tissue: type I, III, IV, V, and VI collagens, laminin, nidogen and heparan sulphate. All samples exhibited similar reactivities to the antibodies, although differences in the intensity and localization of the immunostaining were found that were clearly correlated with between-species differences in integumental ultrastructure. These findings indicate that the composition of the integumental ECM is similar in the three classes of molluscs examined and that several types of collagen are present. However molluscan subepidermal connective tissue differs from the ECM of vertebrate dermis: molluscan integumental ECM contains collagens similar to type I, V and VI collagens but has no type III-similar collagen. Furthermore molecules similar to the type IV collagen, laminin, nidogen and heparan sulphate of vertebrates were present ubiquitously in molluscan basement membrane, confirming the statement that the structure and composition of basement membrane have remained constant throughout the evolution of all animal phyla.

Original languageEnglish
Pages (from-to)259-272
Number of pages14
JournalEuropean journal of histochemistry : EJH
Volume46
Issue number3
Publication statusPublished - 2002

Keywords

  • Collagen
  • Immunohistochemistry
  • Integument
  • Laminin
  • Mollusca
  • Nidogen
  • Proteoglycans

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Animal Science and Zoology
  • Developmental Biology

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