Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation

G. Girolomoni, C. Pincelli, G. Zambruno, M. Andreani, C. Giardini, G. Lucarelli, A. Giannetti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Graft-versus-host disease (GVHD) is an immunologically mediated disease occurring most frequently after allogeneic bone marrow transplantation. The aim of this study was to evaluate the contribution of immunohistochemistry in the diagnosis of cutaneous GVHD. Patients transplanted for either leukemia or β-thalassemia were included in the study. Skin lesions of acute and chronic GVHD were examined both by direct immunofluorescence to detect immunoglobulin deposits and by an avidin-biotin-peroxidase complex technique to evaluate the inflammatory cell infiltrate. Epidermal and dermal fluorescent bodies (IgG and IgM) were frequently found in both acute and chronic GVHD. Most of the infiltrating cells were CD3+T lymphocytes, with CD8+ cells representing the major cell population invading the epidermis both in acute GVHD and in chronic lichenoid GVHD. A small proportion of the dermal cells were CD14+ macrophages; no B cells were detected. HLA-DR, but not HLA-DQ antigens, were variably expressed by keratinocytes in all cases of acute GVHD and in chronic lichenoid GVHD. KL-1, a monoclonal antikeratin antibody specific for the 56.5 KD acidic polypeptide usually present in suprabasal keratinocytes, stained all epidermal layers, including the basal layer. Langerhans cells were dramatically reduced in number in the epidermis of both acute and chronic lichenoid GVHD. It is concluded that immunohistologic analysis may be supportive in the diagnosis of acute and early chronic lichenoid cutaneous GVHD.

Original languageEnglish
Pages (from-to)314-323
Number of pages10
JournalJournal of Dermatology
Volume18
Issue number6
Publication statusPublished - 1991

Fingerprint

Homologous Transplantation
Graft vs Host Disease
Bone Marrow Transplantation
Immunohistochemistry
Skin
Keratinocytes
Epidermis
HLA-DQ Antigens
Direct Fluorescent Antibody Technique
Thalassemia
Langerhans Cells
Avidin
HLA-DR Antigens
Biotin
Peroxidase
Immunoglobulin M
Immunoglobulins
Leukemia
B-Lymphocytes
Immunoglobulin G

ASJC Scopus subject areas

  • Dermatology

Cite this

Girolomoni, G., Pincelli, C., Zambruno, G., Andreani, M., Giardini, C., Lucarelli, G., & Giannetti, A. (1991). Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation. Journal of Dermatology, 18(6), 314-323.

Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation. / Girolomoni, G.; Pincelli, C.; Zambruno, G.; Andreani, M.; Giardini, C.; Lucarelli, G.; Giannetti, A.

In: Journal of Dermatology, Vol. 18, No. 6, 1991, p. 314-323.

Research output: Contribution to journalArticle

Girolomoni, G, Pincelli, C, Zambruno, G, Andreani, M, Giardini, C, Lucarelli, G & Giannetti, A 1991, 'Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation', Journal of Dermatology, vol. 18, no. 6, pp. 314-323.
Girolomoni G, Pincelli C, Zambruno G, Andreani M, Giardini C, Lucarelli G et al. Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation. Journal of Dermatology. 1991;18(6):314-323.
Girolomoni, G. ; Pincelli, C. ; Zambruno, G. ; Andreani, M. ; Giardini, C. ; Lucarelli, G. ; Giannetti, A. / Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation. In: Journal of Dermatology. 1991 ; Vol. 18, No. 6. pp. 314-323.
@article{0e6c8382c94944d487ba1f66a2a4e561,
title = "Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation",
abstract = "Graft-versus-host disease (GVHD) is an immunologically mediated disease occurring most frequently after allogeneic bone marrow transplantation. The aim of this study was to evaluate the contribution of immunohistochemistry in the diagnosis of cutaneous GVHD. Patients transplanted for either leukemia or β-thalassemia were included in the study. Skin lesions of acute and chronic GVHD were examined both by direct immunofluorescence to detect immunoglobulin deposits and by an avidin-biotin-peroxidase complex technique to evaluate the inflammatory cell infiltrate. Epidermal and dermal fluorescent bodies (IgG and IgM) were frequently found in both acute and chronic GVHD. Most of the infiltrating cells were CD3+T lymphocytes, with CD8+ cells representing the major cell population invading the epidermis both in acute GVHD and in chronic lichenoid GVHD. A small proportion of the dermal cells were CD14+ macrophages; no B cells were detected. HLA-DR, but not HLA-DQ antigens, were variably expressed by keratinocytes in all cases of acute GVHD and in chronic lichenoid GVHD. KL-1, a monoclonal antikeratin antibody specific for the 56.5 KD acidic polypeptide usually present in suprabasal keratinocytes, stained all epidermal layers, including the basal layer. Langerhans cells were dramatically reduced in number in the epidermis of both acute and chronic lichenoid GVHD. It is concluded that immunohistologic analysis may be supportive in the diagnosis of acute and early chronic lichenoid cutaneous GVHD.",
author = "G. Girolomoni and C. Pincelli and G. Zambruno and M. Andreani and C. Giardini and G. Lucarelli and A. Giannetti",
year = "1991",
language = "English",
volume = "18",
pages = "314--323",
journal = "Journal of Dermatology",
issn = "0385-2407",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Immunohistochemistry of cutaneous graft-versus-host disease after allogeneic bone marrow transplantation

AU - Girolomoni, G.

AU - Pincelli, C.

AU - Zambruno, G.

AU - Andreani, M.

AU - Giardini, C.

AU - Lucarelli, G.

AU - Giannetti, A.

PY - 1991

Y1 - 1991

N2 - Graft-versus-host disease (GVHD) is an immunologically mediated disease occurring most frequently after allogeneic bone marrow transplantation. The aim of this study was to evaluate the contribution of immunohistochemistry in the diagnosis of cutaneous GVHD. Patients transplanted for either leukemia or β-thalassemia were included in the study. Skin lesions of acute and chronic GVHD were examined both by direct immunofluorescence to detect immunoglobulin deposits and by an avidin-biotin-peroxidase complex technique to evaluate the inflammatory cell infiltrate. Epidermal and dermal fluorescent bodies (IgG and IgM) were frequently found in both acute and chronic GVHD. Most of the infiltrating cells were CD3+T lymphocytes, with CD8+ cells representing the major cell population invading the epidermis both in acute GVHD and in chronic lichenoid GVHD. A small proportion of the dermal cells were CD14+ macrophages; no B cells were detected. HLA-DR, but not HLA-DQ antigens, were variably expressed by keratinocytes in all cases of acute GVHD and in chronic lichenoid GVHD. KL-1, a monoclonal antikeratin antibody specific for the 56.5 KD acidic polypeptide usually present in suprabasal keratinocytes, stained all epidermal layers, including the basal layer. Langerhans cells were dramatically reduced in number in the epidermis of both acute and chronic lichenoid GVHD. It is concluded that immunohistologic analysis may be supportive in the diagnosis of acute and early chronic lichenoid cutaneous GVHD.

AB - Graft-versus-host disease (GVHD) is an immunologically mediated disease occurring most frequently after allogeneic bone marrow transplantation. The aim of this study was to evaluate the contribution of immunohistochemistry in the diagnosis of cutaneous GVHD. Patients transplanted for either leukemia or β-thalassemia were included in the study. Skin lesions of acute and chronic GVHD were examined both by direct immunofluorescence to detect immunoglobulin deposits and by an avidin-biotin-peroxidase complex technique to evaluate the inflammatory cell infiltrate. Epidermal and dermal fluorescent bodies (IgG and IgM) were frequently found in both acute and chronic GVHD. Most of the infiltrating cells were CD3+T lymphocytes, with CD8+ cells representing the major cell population invading the epidermis both in acute GVHD and in chronic lichenoid GVHD. A small proportion of the dermal cells were CD14+ macrophages; no B cells were detected. HLA-DR, but not HLA-DQ antigens, were variably expressed by keratinocytes in all cases of acute GVHD and in chronic lichenoid GVHD. KL-1, a monoclonal antikeratin antibody specific for the 56.5 KD acidic polypeptide usually present in suprabasal keratinocytes, stained all epidermal layers, including the basal layer. Langerhans cells were dramatically reduced in number in the epidermis of both acute and chronic lichenoid GVHD. It is concluded that immunohistologic analysis may be supportive in the diagnosis of acute and early chronic lichenoid cutaneous GVHD.

UR - http://www.scopus.com/inward/record.url?scp=0025883482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025883482&partnerID=8YFLogxK

M3 - Article

C2 - 1939860

AN - SCOPUS:0025883482

VL - 18

SP - 314

EP - 323

JO - Journal of Dermatology

JF - Journal of Dermatology

SN - 0385-2407

IS - 6

ER -