Immunolocalization of sperm protein 17 in human testis and ejaculated spermatozoa

Fabio Grizzi; Maurizio Chiriva-Internati; Barbara Franceschini; Paul L. Hermonat; Giuseppe Soda; Seah H. Lim; Nicola Dioguardi

Immunolocalization of sperm protein 17 in human testis and ejaculated spermatozoa

Fabio Grizzi, Maurizio Chiriva-Internati, Barbara Franceschini, Paul L. Hermonat, Giuseppe Soda, Seah H. Lim, Nicola Dioguardi

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

Sperm protein 17(Sp17) is a highly conserved mammalian protein whose primary function is still poorly understood. Immunohistochemistry (IHC) in the human testis reveals the presence of Sp17 in some spermatocytes and abundantly in spermatids. All spermatogonia, Sertoli cells, and Leydig cells appear to be immunonegative for Sp17, whereas some interstitial cells are immunopositive. IHC recognized two distinct populations (immunopositive or not for Sp17) in the ejaculated spermatozoa. Although it will be necessary to clarify why some ejaculated spermatozoa do not contain Sp17, its distribution suggests that this protein may be associated with some phases of germinal cell differentiation.

Original language	English
Pages (from-to)	1245-1248
Number of pages	4
Journal	Journal of Histochemistry and Cytochemistry
Volume	51
Issue number	9
Publication status	Published - Sep 1 2003

Keywords

Immunohistochemistry
Sperm protein 17
Spermatogenesis
Testis

ASJC Scopus subject areas

Anatomy
Cell Biology

Cite this

Immunolocalization of sperm protein 17 in human testis and ejaculated spermatozoa. / Grizzi, Fabio; Chiriva-Internati, Maurizio; Franceschini, Barbara; Hermonat, Paul L.; Soda, Giuseppe; Lim, Seah H.; Dioguardi, Nicola.

In: Journal of Histochemistry and Cytochemistry, Vol. 51, No. 9, 01.09.2003, p. 1245-1248.

Research output: Contribution to journal › Article › peer-review

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AU - Soda, Giuseppe

AU - Lim, Seah H.

AU - Dioguardi, Nicola

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AB - Sperm protein 17(Sp17) is a highly conserved mammalian protein whose primary function is still poorly understood. Immunohistochemistry (IHC) in the human testis reveals the presence of Sp17 in some spermatocytes and abundantly in spermatids. All spermatogonia, Sertoli cells, and Leydig cells appear to be immunonegative for Sp17, whereas some interstitial cells are immunopositive. IHC recognized two distinct populations (immunopositive or not for Sp17) in the ejaculated spermatozoa. Although it will be necessary to clarify why some ejaculated spermatozoa do not contain Sp17, its distribution suggests that this protein may be associated with some phases of germinal cell differentiation.

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Immunolocalization of sperm protein 17 in human testis and ejaculated spermatozoa

Abstract

Keywords

ASJC Scopus subject areas

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