Immunolocation of lipoteichoic acid on group B streptococcal surface

G. Orefici, A. Molinari, G. Donelli, S. Paradisi, G. Teti, G. Arancia

Research output: Contribution to journalArticle

Abstract

The distribution of lipoteichoic acid (LTA) in a reference strain of group B streptococci was investigated by immuno-electron microscopy. Observations of unfixed and unembedded streptococci incubated with LTA-antibodies and protein A-gold complexes indicate that the lipoteichoic acid is located within the bacterial capsule. Gold particles were distributed all around the cell and apparently aggregated to form filament-like structures. Electron microscopic observations of capsular structure in thin sections of cells embedded after stabilisation with wheat germ agglutinin confirmed the location of LTA molecules within the bacterial capsule.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalFEMS Microbiology Letters
Volume34
Issue number1
Publication statusPublished - 1986

Keywords

  • bacterial adherence
  • immuno-electron microscopy
  • Streptococcus agalactiae

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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  • Cite this

    Orefici, G., Molinari, A., Donelli, G., Paradisi, S., Teti, G., & Arancia, G. (1986). Immunolocation of lipoteichoic acid on group B streptococcal surface. FEMS Microbiology Letters, 34(1), 111-115.