Immunological and clinical impact of cancer stem cells in vulvar cancer: Role of cd133/cd24/abcg2-expressing cells

Chiara Napoletano, Filippo Bellati, Ilary Ruscito, Milena Pernice, Ilaria Grazia Zizzari, Salvatore Caponnetto, Federica Tomao, Luigi Frigerio, Marco Liberati, Aurelia Rughetti, Donatella Caserta, Pierluigi Benedetti Panici, Marianna Nuti

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Cancer stem cells (CSCs) are tumour-initiating cells with self-renewal properties and chemo/radio-resistance. Regulatory T-cells (Tregs) influence CSCs through several mechanisms. In different solid tumours, the presence of both cell populations correlated with poor survival. In vulvar cancer, little is known regarding biological markers able to predict patient prognosis. We investigated the presence and clinical impact of CSCs and infiltrating Treg in primary vulvar cancer. Materials and Methods: Paraffinembedded tissue specimens derived from 43 patients with vulvar cancer were analyzed by immunohistochemistry for the expression of prominin-1 (CD133), CD24, ATP-binding cassette sub-family G member 2 (ABCG2) (CSC markers) and forkhead box protein P3 (FOXP3) (Treg marker). Results: CD133 expression correlated with younger age at diagnosis (p<0.01), lymph-node metastasis (p<0.05) and larger tumour diameter (p<0.05). CD133+ tumours showed a high FOXP3+ T-cell infiltration. Overall survival and progression-free survival were not influenced by the expression of the analyzed biomarkers. Conclusion: In vulvar cancer, CSCs were more frequently expressed in younger aged patients and those with aggressive disease. Their presence was also associated with high Treg infiltration, which contributes to the generation of an immunosuppressive milieu.

Original languageEnglish
Pages (from-to)5109-5116
Number of pages8
JournalAnticancer Research
Volume36
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

Keywords

  • ABCG2
  • Cancer stem cells
  • CD133
  • CD24
  • Vulvar cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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