Immunological basis for IgE hyper-production in enfuvirtide-treated HIV-positive patients

Samuele E. Burastero, Clara Paolucci, Daniela Breda, Armando Soldarini, Fernanda Dorigatti, Elisa Soprana, Hamid Hasson, Priscilla Biswas, Adriano Lazzarin, Antonella Castagna

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We previously reported that enfuvirtide (ENF) treatment is accompanied by a selective increase of serum IgE. We asked whether ENF had intrinsic capability to direct B-lymphocytes to switch to IgE and/or if it could drive CD4 T cells to a Th2 phenotype. ENF was added in vitro: (a) to B-lymphocytes stimulated with IgE-switch inducing stimuli; (b) to peripheral blood mononuclear cells. Total IgE production by B cells and IL4 and IFN-γ production by CD4 T lymphocytes were evaluated, respectively. ENF had no measurable effect on the IgE production by B-lymphocytes. In contrast, it sharply increased the IL4 to IFN-γ (a correlate of the Th2 phenotype) when added in vitro to T cells from healthy donors or from single ENF-treated patients. The hyper-IgE production in ENF-treated patients is associated with the in vitro induction of a type-2 phenotype in CD4 T cells.

Original languageEnglish
Pages (from-to)168-176
Number of pages9
JournalJournal of Clinical Immunology
Volume26
Issue number2
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Immunoglobulin E
HIV
B-Lymphocytes
T-Lymphocytes
Phenotype
Interleukin-4
enfuvirtide
Blood Cells
Tissue Donors
Serum
In Vitro Techniques

Keywords

  • B-lymphocytes
  • Enfuvirtide (ENF)
  • IFN-γ
  • Ig switch
  • IgE, Th1
  • IL-4
  • Immunological response
  • T-lymphocytes
  • Th2

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Immunological basis for IgE hyper-production in enfuvirtide-treated HIV-positive patients. / Burastero, Samuele E.; Paolucci, Clara; Breda, Daniela; Soldarini, Armando; Dorigatti, Fernanda; Soprana, Elisa; Hasson, Hamid; Biswas, Priscilla; Lazzarin, Adriano; Castagna, Antonella.

In: Journal of Clinical Immunology, Vol. 26, No. 2, 03.2006, p. 168-176.

Research output: Contribution to journalArticle

Burastero, Samuele E. ; Paolucci, Clara ; Breda, Daniela ; Soldarini, Armando ; Dorigatti, Fernanda ; Soprana, Elisa ; Hasson, Hamid ; Biswas, Priscilla ; Lazzarin, Adriano ; Castagna, Antonella. / Immunological basis for IgE hyper-production in enfuvirtide-treated HIV-positive patients. In: Journal of Clinical Immunology. 2006 ; Vol. 26, No. 2. pp. 168-176.
@article{70d5c0fb7ec44580b50dccef9f86d9bc,
title = "Immunological basis for IgE hyper-production in enfuvirtide-treated HIV-positive patients",
abstract = "We previously reported that enfuvirtide (ENF) treatment is accompanied by a selective increase of serum IgE. We asked whether ENF had intrinsic capability to direct B-lymphocytes to switch to IgE and/or if it could drive CD4 T cells to a Th2 phenotype. ENF was added in vitro: (a) to B-lymphocytes stimulated with IgE-switch inducing stimuli; (b) to peripheral blood mononuclear cells. Total IgE production by B cells and IL4 and IFN-γ production by CD4 T lymphocytes were evaluated, respectively. ENF had no measurable effect on the IgE production by B-lymphocytes. In contrast, it sharply increased the IL4 to IFN-γ (a correlate of the Th2 phenotype) when added in vitro to T cells from healthy donors or from single ENF-treated patients. The hyper-IgE production in ENF-treated patients is associated with the in vitro induction of a type-2 phenotype in CD4 T cells.",
keywords = "B-lymphocytes, Enfuvirtide (ENF), IFN-γ, Ig switch, IgE, Th1, IL-4, Immunological response, T-lymphocytes, Th2",
author = "Burastero, {Samuele E.} and Clara Paolucci and Daniela Breda and Armando Soldarini and Fernanda Dorigatti and Elisa Soprana and Hamid Hasson and Priscilla Biswas and Adriano Lazzarin and Antonella Castagna",
year = "2006",
month = "3",
doi = "10.1007/s10875-006-9005-7",
language = "English",
volume = "26",
pages = "168--176",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Immunological basis for IgE hyper-production in enfuvirtide-treated HIV-positive patients

AU - Burastero, Samuele E.

AU - Paolucci, Clara

AU - Breda, Daniela

AU - Soldarini, Armando

AU - Dorigatti, Fernanda

AU - Soprana, Elisa

AU - Hasson, Hamid

AU - Biswas, Priscilla

AU - Lazzarin, Adriano

AU - Castagna, Antonella

PY - 2006/3

Y1 - 2006/3

N2 - We previously reported that enfuvirtide (ENF) treatment is accompanied by a selective increase of serum IgE. We asked whether ENF had intrinsic capability to direct B-lymphocytes to switch to IgE and/or if it could drive CD4 T cells to a Th2 phenotype. ENF was added in vitro: (a) to B-lymphocytes stimulated with IgE-switch inducing stimuli; (b) to peripheral blood mononuclear cells. Total IgE production by B cells and IL4 and IFN-γ production by CD4 T lymphocytes were evaluated, respectively. ENF had no measurable effect on the IgE production by B-lymphocytes. In contrast, it sharply increased the IL4 to IFN-γ (a correlate of the Th2 phenotype) when added in vitro to T cells from healthy donors or from single ENF-treated patients. The hyper-IgE production in ENF-treated patients is associated with the in vitro induction of a type-2 phenotype in CD4 T cells.

AB - We previously reported that enfuvirtide (ENF) treatment is accompanied by a selective increase of serum IgE. We asked whether ENF had intrinsic capability to direct B-lymphocytes to switch to IgE and/or if it could drive CD4 T cells to a Th2 phenotype. ENF was added in vitro: (a) to B-lymphocytes stimulated with IgE-switch inducing stimuli; (b) to peripheral blood mononuclear cells. Total IgE production by B cells and IL4 and IFN-γ production by CD4 T lymphocytes were evaluated, respectively. ENF had no measurable effect on the IgE production by B-lymphocytes. In contrast, it sharply increased the IL4 to IFN-γ (a correlate of the Th2 phenotype) when added in vitro to T cells from healthy donors or from single ENF-treated patients. The hyper-IgE production in ENF-treated patients is associated with the in vitro induction of a type-2 phenotype in CD4 T cells.

KW - B-lymphocytes

KW - Enfuvirtide (ENF)

KW - IFN-γ

KW - Ig switch

KW - IgE, Th1

KW - IL-4

KW - Immunological response

KW - T-lymphocytes

KW - Th2

UR - http://www.scopus.com/inward/record.url?scp=33745007159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745007159&partnerID=8YFLogxK

U2 - 10.1007/s10875-006-9005-7

DO - 10.1007/s10875-006-9005-7

M3 - Article

C2 - 16602031

AN - SCOPUS:33745007159

VL - 26

SP - 168

EP - 176

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

SN - 0271-9142

IS - 2

ER -