Immunological Profile of Silent Brain Infarction and Lacunar Stroke

Paola Sarchielli, Katiuscia Nardi, Davide Chiasserini, Paolo Eusebi, Michela Tantucci, Vittorio Di Piero, Marta Altieri, Carmine Marini, Tommasina Russo, Mauro Silvestrini, Isabella Paolino, Paolo Calabresi, Lucilla Parnetti

Research output: Contribution to journalArticle

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Abstract

Neuroinflammation is believed to be involved in the pathophysiological mechanisms of silent brain infarcts (SBI). However, the immunological profile of SBI has been scarcely investigated. In the context of a national research project named SILENCE, aimed at investigating clinical, biochemical and pathogenic features of SBI, we have measured the plasma profile of some inflammatory-related molecules in SBI patients (n = 21), patients with recent lacunar infarcts (LI, n = 28) and healthy controls (n = 31), consecutively enrolled in four Italian centres. A panel of chemokines (MIG, CTACK, IL16, SDF1a, MCP1), growth factors (SCF, SCGFb, HGF, IL3), immunoglobulin-type adhesion molecules (ICAM1, VCAM1), proinflammatory cytokines (IL18, INFa2, MIF, IL12p40), cell surface receptors on T-cells (IL2Ra), and inductors of apoptosis (TRAIL) was assessed in plasma samples by Luminex xMAP™ technology. Immunological parameters were compared using non-parametric statistics and performance to distinguish SBI and LI was evaluated by receiver operating characteristic (ROC) analysis. Plasma levels of ICAM1 were significantly higher in both SBI and LI patients as compared to controls (SBI≥LI>Ctrl). A different trend was observed for IL16 (SBICtrl), SCF (LICtrl) and SCGFb (SBI>LICtrl) and IL18 when compared to LI patients (Ctrl≤SBI>LI). All the other immunological markers did not significantly differ among groups. According to ROC analysis, the best predictor for SBI condition was the chemokine MIG (AUC = 0.84, sensitivity 86%, specificity 77%), while SCF had the best performance in distinguishing LI patients (AUC = 0.84, sensitivity 86%, specificity 68%). These results confirm the involvement of inflammatory processes in cerebrovascular disorders, particularly in SBI, a very common age-related condition. The differences in plasma profile of inflammatory molecules may underlie different pathological mechanisms in SBI and LI patients.

Original languageEnglish
Article numbere68428
JournalPLoS One
Volume8
Issue number7
DOIs
Publication statusPublished - Jul 9 2013

Fingerprint

Brain Infarction
Lacunar Stroke
infarction
stroke
Brain
brain
Interleukin-16
Plasmas
Interleukin-18
Chemokines
ROC Curve
Chemokine CCL27
Molecules
Area Under Curve
Cerebrovascular Disorders
Sensitivity and Specificity
cerebrovascular disorders
T-cells
Cell Surface Receptors
Nonparametric Statistics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sarchielli, P., Nardi, K., Chiasserini, D., Eusebi, P., Tantucci, M., Di Piero, V., ... Parnetti, L. (2013). Immunological Profile of Silent Brain Infarction and Lacunar Stroke. PLoS One, 8(7), [e68428]. https://doi.org/10.1371/journal.pone.0068428

Immunological Profile of Silent Brain Infarction and Lacunar Stroke. / Sarchielli, Paola; Nardi, Katiuscia; Chiasserini, Davide; Eusebi, Paolo; Tantucci, Michela; Di Piero, Vittorio; Altieri, Marta; Marini, Carmine; Russo, Tommasina; Silvestrini, Mauro; Paolino, Isabella; Calabresi, Paolo; Parnetti, Lucilla.

In: PLoS One, Vol. 8, No. 7, e68428, 09.07.2013.

Research output: Contribution to journalArticle

Sarchielli, P, Nardi, K, Chiasserini, D, Eusebi, P, Tantucci, M, Di Piero, V, Altieri, M, Marini, C, Russo, T, Silvestrini, M, Paolino, I, Calabresi, P & Parnetti, L 2013, 'Immunological Profile of Silent Brain Infarction and Lacunar Stroke', PLoS One, vol. 8, no. 7, e68428. https://doi.org/10.1371/journal.pone.0068428
Sarchielli P, Nardi K, Chiasserini D, Eusebi P, Tantucci M, Di Piero V et al. Immunological Profile of Silent Brain Infarction and Lacunar Stroke. PLoS One. 2013 Jul 9;8(7). e68428. https://doi.org/10.1371/journal.pone.0068428
Sarchielli, Paola ; Nardi, Katiuscia ; Chiasserini, Davide ; Eusebi, Paolo ; Tantucci, Michela ; Di Piero, Vittorio ; Altieri, Marta ; Marini, Carmine ; Russo, Tommasina ; Silvestrini, Mauro ; Paolino, Isabella ; Calabresi, Paolo ; Parnetti, Lucilla. / Immunological Profile of Silent Brain Infarction and Lacunar Stroke. In: PLoS One. 2013 ; Vol. 8, No. 7.
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