Immunomodulatory properties of porcine, bone marrow-derived multipotent mesenchymal stromal cells and comparison with their human counterpart.

P. Comite, L. Cobianchi, M. A. Avanzini, M. Mantelli, V. Achille, S. Zonta, C. Ferrari, M. Alessiani, A. De Silvestri, G. M. Gandolfo, L. Inverardi, L. Brescia, A. Pietrabissa, P. Dionigi, F. Locatelli, M. E. Bernardo

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Abstract

Thanks to their immunonodulatory properties, multipotent mesenchymal stromal cells (MSCs) are a promising strategy for preventing/reducing the risk of graft rejection after hematopoietic cell and solid organ transplantation. We have previously demonstrated that porcine MSCs (pMSCs) can be isolated from bone marrow and display similar morphology and differentiative capacity as compared to human MSC (hMSCs). In this study, we investigated the in vitro immunomodulatory properties (namely the ability to suppress lymphocyte proliferation in response to phytohemagglutinin and the cytokine production in the culture supernatants) of pMSCs from six Large White 6-month old piglets. Similarly to hMSCs, pMSCs reduced the phytohemagglutinin-induced lymphocyte proliferation. High levels of IL-6 were found in culture supernatants, whereas IL-10 and TGF-β were not detectable. In conclusion, ex vivo expanded pMSCs share selected biological/functional properties with hMSCs. pMSCs may be used in in vivo models to investigate novel approaches of prevention of graft rejection in solid organ transplantation.

Original languageEnglish
JournalCellular and Molecular Biology
VolumeSuppl 57
Publication statusPublished - 2011

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Transplantation (surgical)
Lymphocytes
Phytohemagglutinins
Mesenchymal Stromal Cells
Grafts
Bone
Swine
Bone Marrow
Interleukin-10
Graft Rejection
Organ Transplantation
Interleukin-6
Cells
Cytokines

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{c9c700e2e7ab4db4a59eddcec49a5fc7,
title = "Immunomodulatory properties of porcine, bone marrow-derived multipotent mesenchymal stromal cells and comparison with their human counterpart.",
abstract = "Thanks to their immunonodulatory properties, multipotent mesenchymal stromal cells (MSCs) are a promising strategy for preventing/reducing the risk of graft rejection after hematopoietic cell and solid organ transplantation. We have previously demonstrated that porcine MSCs (pMSCs) can be isolated from bone marrow and display similar morphology and differentiative capacity as compared to human MSC (hMSCs). In this study, we investigated the in vitro immunomodulatory properties (namely the ability to suppress lymphocyte proliferation in response to phytohemagglutinin and the cytokine production in the culture supernatants) of pMSCs from six Large White 6-month old piglets. Similarly to hMSCs, pMSCs reduced the phytohemagglutinin-induced lymphocyte proliferation. High levels of IL-6 were found in culture supernatants, whereas IL-10 and TGF-β were not detectable. In conclusion, ex vivo expanded pMSCs share selected biological/functional properties with hMSCs. pMSCs may be used in in vivo models to investigate novel approaches of prevention of graft rejection in solid organ transplantation.",
author = "P. Comite and L. Cobianchi and Avanzini, {M. A.} and M. Mantelli and V. Achille and S. Zonta and C. Ferrari and M. Alessiani and {De Silvestri}, A. and Gandolfo, {G. M.} and L. Inverardi and L. Brescia and A. Pietrabissa and P. Dionigi and F. Locatelli and Bernardo, {M. E.}",
year = "2011",
language = "English",
volume = "Suppl 57",
journal = "Cellular and Molecular Biology",
issn = "0145-5680",
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TY - JOUR

T1 - Immunomodulatory properties of porcine, bone marrow-derived multipotent mesenchymal stromal cells and comparison with their human counterpart.

AU - Comite, P.

AU - Cobianchi, L.

AU - Avanzini, M. A.

AU - Mantelli, M.

AU - Achille, V.

AU - Zonta, S.

AU - Ferrari, C.

AU - Alessiani, M.

AU - De Silvestri, A.

AU - Gandolfo, G. M.

AU - Inverardi, L.

AU - Brescia, L.

AU - Pietrabissa, A.

AU - Dionigi, P.

AU - Locatelli, F.

AU - Bernardo, M. E.

PY - 2011

Y1 - 2011

N2 - Thanks to their immunonodulatory properties, multipotent mesenchymal stromal cells (MSCs) are a promising strategy for preventing/reducing the risk of graft rejection after hematopoietic cell and solid organ transplantation. We have previously demonstrated that porcine MSCs (pMSCs) can be isolated from bone marrow and display similar morphology and differentiative capacity as compared to human MSC (hMSCs). In this study, we investigated the in vitro immunomodulatory properties (namely the ability to suppress lymphocyte proliferation in response to phytohemagglutinin and the cytokine production in the culture supernatants) of pMSCs from six Large White 6-month old piglets. Similarly to hMSCs, pMSCs reduced the phytohemagglutinin-induced lymphocyte proliferation. High levels of IL-6 were found in culture supernatants, whereas IL-10 and TGF-β were not detectable. In conclusion, ex vivo expanded pMSCs share selected biological/functional properties with hMSCs. pMSCs may be used in in vivo models to investigate novel approaches of prevention of graft rejection in solid organ transplantation.

AB - Thanks to their immunonodulatory properties, multipotent mesenchymal stromal cells (MSCs) are a promising strategy for preventing/reducing the risk of graft rejection after hematopoietic cell and solid organ transplantation. We have previously demonstrated that porcine MSCs (pMSCs) can be isolated from bone marrow and display similar morphology and differentiative capacity as compared to human MSC (hMSCs). In this study, we investigated the in vitro immunomodulatory properties (namely the ability to suppress lymphocyte proliferation in response to phytohemagglutinin and the cytokine production in the culture supernatants) of pMSCs from six Large White 6-month old piglets. Similarly to hMSCs, pMSCs reduced the phytohemagglutinin-induced lymphocyte proliferation. High levels of IL-6 were found in culture supernatants, whereas IL-10 and TGF-β were not detectable. In conclusion, ex vivo expanded pMSCs share selected biological/functional properties with hMSCs. pMSCs may be used in in vivo models to investigate novel approaches of prevention of graft rejection in solid organ transplantation.

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