Immunonutrition before esophagectomy: Impact on immune surveillance mechanisms

Marco Scarpa, Andromachi Kotsafti, Matteo Fassan, Melania Scarpa, Francesco Cavallin, Teresa Nardi, Eleonora Pinto, Rita Alfieri, Matteo Cagol, Marco Agostini, Marco Agostini, Massimo Rugge, Ignazio Castagliuolo, Carlo Castoro

Research output: Contribution to journalArticle

Abstract

Preoperative oral immunonutrition was demonstrated to improve immune response and to decrease the infection rate in patients with cancer. This study aimed to assess how immunonutrition could influence the immune cell response in the mucosal microenvironment of esophageal adenocarcinoma. Therefore, A prospective cohort of consecutive patients undergoing esophagectomy for esophageal adenocarcinoma was enrolled. A subgroup of them was given preoperative oral immunonutrition with Oral Impact® and was compared to those who received no preoperative supplementation. Mucosal samples from healthy esophagus were obtained at esophagectomy. Histology, immunohistochemistry, gene expression analysis, and cytofluorimetry were performed. Markers of activation of antigen-presenting cells (CD80, CD86, and HLA-I), innate immunity (TLR4 and MyD88), and cytotoxic lymphocyte infiltration and activation (CD8, CD38, CD69, and CD107) were measured. In all, 50 patients received preoperative Oral Impact® and 129 patients received no nutritional support. CD80, CD86, MyD88, and CD69 messenger RNA expression was significantly increased in patients receiving immunonutrition compared to controls. In the subgroup of patients with stages I-II cancer, the rate of epithelial cells expressing CD80 and HLA-ABC was significantly higher in those receiving immunonutrition compared to controls as well as CD8+ CD28+ cell rate. Immunonutrition administration before surgery was significantly associated to increased degranulating CD8 and natural killer cells (CD107+) infiltrating the healthy esophageal mucosa. All the comparisons were adjusted for cancer stage and preoperative therapy. In conclusion, in healthy esophageal mucosa of patients undergoing esophagectomy, a 5-day course of immunonutrition enhances expression of antigen-presenting cells activity and increased CD8+ T cell activation and degranulating activity. Further studies are warranted to understand the clinical implication in terms of cancer recurrence.
Original languageEnglish
Article number1010428317728683
JournalTumor Biology
Volume39
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Esophagectomy
Antigen-Presenting Cells
Neoplasms
Adenocarcinoma
Nutritional Support
Differentiation Antigens
Lymphocyte Activation
Innate Immunity
Natural Killer Cells
Esophagus
Histology
Epithelial Cells
Immunohistochemistry
T-Lymphocytes
Gene Expression
Recurrence
Messenger RNA
Infection

Keywords

  • CD8
  • CD80
  • esophageal adenocarcinoma
  • Immunonutrition
  • immunosurveillance

Cite this

Immunonutrition before esophagectomy: Impact on immune surveillance mechanisms. / Scarpa, Marco; Kotsafti, Andromachi; Fassan, Matteo; Scarpa, Melania; Cavallin, Francesco; Nardi, Teresa; Pinto, Eleonora; Alfieri, Rita; Cagol, Matteo; Agostini, Marco; Agostini, Marco; Rugge, Massimo; Castagliuolo, Ignazio; Castoro, Carlo.

In: Tumor Biology, Vol. 39, No. 10, 1010428317728683, 01.10.2017.

Research output: Contribution to journalArticle

@article{b4b7ae8a09f84085b2e1eeb2c1c7486c,
title = "Immunonutrition before esophagectomy: Impact on immune surveillance mechanisms",
abstract = "Preoperative oral immunonutrition was demonstrated to improve immune response and to decrease the infection rate in patients with cancer. This study aimed to assess how immunonutrition could influence the immune cell response in the mucosal microenvironment of esophageal adenocarcinoma. Therefore, A prospective cohort of consecutive patients undergoing esophagectomy for esophageal adenocarcinoma was enrolled. A subgroup of them was given preoperative oral immunonutrition with Oral Impact{\circledR} and was compared to those who received no preoperative supplementation. Mucosal samples from healthy esophagus were obtained at esophagectomy. Histology, immunohistochemistry, gene expression analysis, and cytofluorimetry were performed. Markers of activation of antigen-presenting cells (CD80, CD86, and HLA-I), innate immunity (TLR4 and MyD88), and cytotoxic lymphocyte infiltration and activation (CD8, CD38, CD69, and CD107) were measured. In all, 50 patients received preoperative Oral Impact{\circledR} and 129 patients received no nutritional support. CD80, CD86, MyD88, and CD69 messenger RNA expression was significantly increased in patients receiving immunonutrition compared to controls. In the subgroup of patients with stages I-II cancer, the rate of epithelial cells expressing CD80 and HLA-ABC was significantly higher in those receiving immunonutrition compared to controls as well as CD8+ CD28+ cell rate. Immunonutrition administration before surgery was significantly associated to increased degranulating CD8 and natural killer cells (CD107+) infiltrating the healthy esophageal mucosa. All the comparisons were adjusted for cancer stage and preoperative therapy. In conclusion, in healthy esophageal mucosa of patients undergoing esophagectomy, a 5-day course of immunonutrition enhances expression of antigen-presenting cells activity and increased CD8+ T cell activation and degranulating activity. Further studies are warranted to understand the clinical implication in terms of cancer recurrence.",
keywords = "CD8, CD80, esophageal adenocarcinoma, Immunonutrition, immunosurveillance",
author = "Marco Scarpa and Andromachi Kotsafti and Matteo Fassan and Melania Scarpa and Francesco Cavallin and Teresa Nardi and Eleonora Pinto and Rita Alfieri and Matteo Cagol and Marco Agostini and Marco Agostini and Massimo Rugge and Ignazio Castagliuolo and Carlo Castoro",
year = "2017",
month = "10",
day = "1",
doi = "10.1177/1010428317728683",
language = "English",
volume = "39",
journal = "Tumor Biology",
issn = "1010-4283",
publisher = "Springer Netherlands",
number = "10",

}

TY - JOUR

T1 - Immunonutrition before esophagectomy: Impact on immune surveillance mechanisms

AU - Scarpa, Marco

AU - Kotsafti, Andromachi

AU - Fassan, Matteo

AU - Scarpa, Melania

AU - Cavallin, Francesco

AU - Nardi, Teresa

AU - Pinto, Eleonora

AU - Alfieri, Rita

AU - Cagol, Matteo

AU - Agostini, Marco

AU - Agostini, Marco

AU - Rugge, Massimo

AU - Castagliuolo, Ignazio

AU - Castoro, Carlo

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Preoperative oral immunonutrition was demonstrated to improve immune response and to decrease the infection rate in patients with cancer. This study aimed to assess how immunonutrition could influence the immune cell response in the mucosal microenvironment of esophageal adenocarcinoma. Therefore, A prospective cohort of consecutive patients undergoing esophagectomy for esophageal adenocarcinoma was enrolled. A subgroup of them was given preoperative oral immunonutrition with Oral Impact® and was compared to those who received no preoperative supplementation. Mucosal samples from healthy esophagus were obtained at esophagectomy. Histology, immunohistochemistry, gene expression analysis, and cytofluorimetry were performed. Markers of activation of antigen-presenting cells (CD80, CD86, and HLA-I), innate immunity (TLR4 and MyD88), and cytotoxic lymphocyte infiltration and activation (CD8, CD38, CD69, and CD107) were measured. In all, 50 patients received preoperative Oral Impact® and 129 patients received no nutritional support. CD80, CD86, MyD88, and CD69 messenger RNA expression was significantly increased in patients receiving immunonutrition compared to controls. In the subgroup of patients with stages I-II cancer, the rate of epithelial cells expressing CD80 and HLA-ABC was significantly higher in those receiving immunonutrition compared to controls as well as CD8+ CD28+ cell rate. Immunonutrition administration before surgery was significantly associated to increased degranulating CD8 and natural killer cells (CD107+) infiltrating the healthy esophageal mucosa. All the comparisons were adjusted for cancer stage and preoperative therapy. In conclusion, in healthy esophageal mucosa of patients undergoing esophagectomy, a 5-day course of immunonutrition enhances expression of antigen-presenting cells activity and increased CD8+ T cell activation and degranulating activity. Further studies are warranted to understand the clinical implication in terms of cancer recurrence.

AB - Preoperative oral immunonutrition was demonstrated to improve immune response and to decrease the infection rate in patients with cancer. This study aimed to assess how immunonutrition could influence the immune cell response in the mucosal microenvironment of esophageal adenocarcinoma. Therefore, A prospective cohort of consecutive patients undergoing esophagectomy for esophageal adenocarcinoma was enrolled. A subgroup of them was given preoperative oral immunonutrition with Oral Impact® and was compared to those who received no preoperative supplementation. Mucosal samples from healthy esophagus were obtained at esophagectomy. Histology, immunohistochemistry, gene expression analysis, and cytofluorimetry were performed. Markers of activation of antigen-presenting cells (CD80, CD86, and HLA-I), innate immunity (TLR4 and MyD88), and cytotoxic lymphocyte infiltration and activation (CD8, CD38, CD69, and CD107) were measured. In all, 50 patients received preoperative Oral Impact® and 129 patients received no nutritional support. CD80, CD86, MyD88, and CD69 messenger RNA expression was significantly increased in patients receiving immunonutrition compared to controls. In the subgroup of patients with stages I-II cancer, the rate of epithelial cells expressing CD80 and HLA-ABC was significantly higher in those receiving immunonutrition compared to controls as well as CD8+ CD28+ cell rate. Immunonutrition administration before surgery was significantly associated to increased degranulating CD8 and natural killer cells (CD107+) infiltrating the healthy esophageal mucosa. All the comparisons were adjusted for cancer stage and preoperative therapy. In conclusion, in healthy esophageal mucosa of patients undergoing esophagectomy, a 5-day course of immunonutrition enhances expression of antigen-presenting cells activity and increased CD8+ T cell activation and degranulating activity. Further studies are warranted to understand the clinical implication in terms of cancer recurrence.

KW - CD8

KW - CD80

KW - esophageal adenocarcinoma

KW - Immunonutrition

KW - immunosurveillance

UR - http://www.scopus.com/inward/record.url?scp=85031750273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031750273&partnerID=8YFLogxK

U2 - 10.1177/1010428317728683

DO - 10.1177/1010428317728683

M3 - Article

VL - 39

JO - Tumor Biology

JF - Tumor Biology

SN - 1010-4283

IS - 10

M1 - 1010428317728683

ER -