TY - JOUR
T1 - Immunophenotype of adult and childhood acute promyelocytic leukaemia
T2 - Correlation with morphology, type of PML gene breakpoint and clinical outcome. A cooperative Italian study on 196 cases
AU - Guglielmi, Cesare
AU - Martelli, Maria Paola
AU - Diverio, Daniela
AU - Fenu, Susanna
AU - Vegna, Maria Luce
AU - Cantù-Rajnoldi, Angelo
AU - Biondi, Andrea
AU - Cocito, Maria Grazia
AU - Del Vecchio, Luigi
AU - Tabilio, Antonio
AU - Avvisati, Giuseppe
AU - Basso, Giuseppe
AU - Lo Coco, Francesco
PY - 1998
Y1 - 1998
N2 - Acute promyelocytic leukaemia (APL), characterized by a specific PML- RARα fusion gene resulting from translocation t(15;17) and by a high response rate to differentiation therapy with all-trans retinoic acid, presents clinical (varying WBC counts, age and treatment outcome), morphological (hypergranular M3 and hypogranular M3V) and molecular (three isoforms of PML breakpoint) heterogeneity. We correlated leukaemic immunophenotype with these aspects in 196 molecularly confirmed APLs (63 children and 133 adults) in Italy. The bcr3 isoform (P=0.05) and FAB M3V (P=0.05) were more frequent in children. We confirmed in APL an immunophenotype characterized by frequent expression of CD13, CD33 and CD9 and rare expression of HLA-DR, CD10, CD7 and CD11b. However, we recognized CD2 in 28%, CD34 in 23% and CD19 in 11% of cases and demonstrated by double labelling that CD34 and CD2 may be co-expressed. CD2, CD34 and CD19 were significantly intercorrelated, and variably associated to other features: CD2 and CD34 with PML bcr3 (P
AB - Acute promyelocytic leukaemia (APL), characterized by a specific PML- RARα fusion gene resulting from translocation t(15;17) and by a high response rate to differentiation therapy with all-trans retinoic acid, presents clinical (varying WBC counts, age and treatment outcome), morphological (hypergranular M3 and hypogranular M3V) and molecular (three isoforms of PML breakpoint) heterogeneity. We correlated leukaemic immunophenotype with these aspects in 196 molecularly confirmed APLs (63 children and 133 adults) in Italy. The bcr3 isoform (P=0.05) and FAB M3V (P=0.05) were more frequent in children. We confirmed in APL an immunophenotype characterized by frequent expression of CD13, CD33 and CD9 and rare expression of HLA-DR, CD10, CD7 and CD11b. However, we recognized CD2 in 28%, CD34 in 23% and CD19 in 11% of cases and demonstrated by double labelling that CD34 and CD2 may be co-expressed. CD2, CD34 and CD19 were significantly intercorrelated, and variably associated to other features: CD2 and CD34 with PML bcr3 (P
KW - Acute promyelocytic leukaemia
KW - CD2
KW - Immunophenotype
KW - PML
KW - Prognosis
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U2 - 10.1046/j.1365-2141.1998.00871.x
DO - 10.1046/j.1365-2141.1998.00871.x
M3 - Article
C2 - 9734655
AN - SCOPUS:0344336086
VL - 102
SP - 1035
EP - 1041
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 4
ER -