Immunophenotype-related microRNA expression in canine chronic lymphocytic leukemia

G. Gioia, M. Mortarino, M. E. Gelain, F. Albonico, E. Ciusani, I. Forno, L. Marconato, V. Martini, S. Comazzi

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) are posttranscriptional regulatory noncoding RNAs used to profile human hematopoietic tumors. In this study, some mature miRNAs was quantitated in peripheral blood from dogs with chronic lymphocytic leukemia (CLL). Relative expression data were normalised against four endogenous controls (let-7a, miR-17-5p, miR-26b, and miR-223) selected by geNorm analysis. The results revealed distinct miRNA patterns in CLL depending on the immunophenotype. Also in dogs, the different miRNAs expression could reflect developmental lineage and tumor differentiation. The similar genetics, physiology and exposure to environment in dogs and humans make the miRNA expression study in canine CLL attractive for comparative oncology.

Original languageEnglish
Pages (from-to)228-235
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume142
Issue number3-4
DOIs
Publication statusPublished - Aug 15 2011

Fingerprint

lymphocytic leukemia
B-Cell Chronic Lymphocytic Leukemia
MicroRNAs
microRNA
Canidae
dogs
Dogs
Untranslated RNA
neoplasms
Neoplasms
physiology
blood

Keywords

  • Chronic lymphocytic leukemia
  • Dog
  • Immunophenotype
  • MicroRNA
  • Quantitative RT PCR
  • Whole blood

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Immunophenotype-related microRNA expression in canine chronic lymphocytic leukemia. / Gioia, G.; Mortarino, M.; Gelain, M. E.; Albonico, F.; Ciusani, E.; Forno, I.; Marconato, L.; Martini, V.; Comazzi, S.

In: Veterinary Immunology and Immunopathology, Vol. 142, No. 3-4, 15.08.2011, p. 228-235.

Research output: Contribution to journalArticle

Gioia, G, Mortarino, M, Gelain, ME, Albonico, F, Ciusani, E, Forno, I, Marconato, L, Martini, V & Comazzi, S 2011, 'Immunophenotype-related microRNA expression in canine chronic lymphocytic leukemia', Veterinary Immunology and Immunopathology, vol. 142, no. 3-4, pp. 228-235. https://doi.org/10.1016/j.vetimm.2011.05.020
Gioia, G. ; Mortarino, M. ; Gelain, M. E. ; Albonico, F. ; Ciusani, E. ; Forno, I. ; Marconato, L. ; Martini, V. ; Comazzi, S. / Immunophenotype-related microRNA expression in canine chronic lymphocytic leukemia. In: Veterinary Immunology and Immunopathology. 2011 ; Vol. 142, No. 3-4. pp. 228-235.
@article{80b11f53693045b8ae541732da6e1af5,
title = "Immunophenotype-related microRNA expression in canine chronic lymphocytic leukemia",
abstract = "MicroRNAs (miRNAs) are posttranscriptional regulatory noncoding RNAs used to profile human hematopoietic tumors. In this study, some mature miRNAs was quantitated in peripheral blood from dogs with chronic lymphocytic leukemia (CLL). Relative expression data were normalised against four endogenous controls (let-7a, miR-17-5p, miR-26b, and miR-223) selected by geNorm analysis. The results revealed distinct miRNA patterns in CLL depending on the immunophenotype. Also in dogs, the different miRNAs expression could reflect developmental lineage and tumor differentiation. The similar genetics, physiology and exposure to environment in dogs and humans make the miRNA expression study in canine CLL attractive for comparative oncology.",
keywords = "Chronic lymphocytic leukemia, Dog, Immunophenotype, MicroRNA, Quantitative RT PCR, Whole blood",
author = "G. Gioia and M. Mortarino and Gelain, {M. E.} and F. Albonico and E. Ciusani and I. Forno and L. Marconato and V. Martini and S. Comazzi",
year = "2011",
month = "8",
day = "15",
doi = "10.1016/j.vetimm.2011.05.020",
language = "English",
volume = "142",
pages = "228--235",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Immunophenotype-related microRNA expression in canine chronic lymphocytic leukemia

AU - Gioia, G.

AU - Mortarino, M.

AU - Gelain, M. E.

AU - Albonico, F.

AU - Ciusani, E.

AU - Forno, I.

AU - Marconato, L.

AU - Martini, V.

AU - Comazzi, S.

PY - 2011/8/15

Y1 - 2011/8/15

N2 - MicroRNAs (miRNAs) are posttranscriptional regulatory noncoding RNAs used to profile human hematopoietic tumors. In this study, some mature miRNAs was quantitated in peripheral blood from dogs with chronic lymphocytic leukemia (CLL). Relative expression data were normalised against four endogenous controls (let-7a, miR-17-5p, miR-26b, and miR-223) selected by geNorm analysis. The results revealed distinct miRNA patterns in CLL depending on the immunophenotype. Also in dogs, the different miRNAs expression could reflect developmental lineage and tumor differentiation. The similar genetics, physiology and exposure to environment in dogs and humans make the miRNA expression study in canine CLL attractive for comparative oncology.

AB - MicroRNAs (miRNAs) are posttranscriptional regulatory noncoding RNAs used to profile human hematopoietic tumors. In this study, some mature miRNAs was quantitated in peripheral blood from dogs with chronic lymphocytic leukemia (CLL). Relative expression data were normalised against four endogenous controls (let-7a, miR-17-5p, miR-26b, and miR-223) selected by geNorm analysis. The results revealed distinct miRNA patterns in CLL depending on the immunophenotype. Also in dogs, the different miRNAs expression could reflect developmental lineage and tumor differentiation. The similar genetics, physiology and exposure to environment in dogs and humans make the miRNA expression study in canine CLL attractive for comparative oncology.

KW - Chronic lymphocytic leukemia

KW - Dog

KW - Immunophenotype

KW - MicroRNA

KW - Quantitative RT PCR

KW - Whole blood

UR - http://www.scopus.com/inward/record.url?scp=79960053680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960053680&partnerID=8YFLogxK

U2 - 10.1016/j.vetimm.2011.05.020

DO - 10.1016/j.vetimm.2011.05.020

M3 - Article

C2 - 21663977

AN - SCOPUS:79960053680

VL - 142

SP - 228

EP - 235

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 3-4

ER -