Immunophenotypic and molecular genetic characterization of a case of CD8+ B cell chronic lymphocytic leukemia

V. Attadia; M. Alosi; S. Improta; M. Baccarani; P. De Paoli

Immunophenotypic and molecular genetic characterization of a case of CD8+ B cell chronic lymphocytic leukemia

V. Attadia, M. Alosi, S. Improta, M. Baccarani, P. De Paoli

Centro di Riferimento Oncologico

Research output: Contribution to journal › Article › peer-review

Abstract

We report the case of a patient with chronic B lymphocytic leukemia, with stable clinical and hematological conditions in the absence of any treatment. The flow cytometry analysis of the patient's cells revealed a CD19⁺, CD5⁺, IgM⁺, IgD⁺, λ chain⁺ phenotype, along with an unusual expression of CD8α/α homo dimer. B cells did not stain with the anti CD8β monoclonal antibody T8/2T8 5H7. Molecular biology analyses confirmed the monoclonal rearrangements of immunoglobulin heavy and λ light chain genes in the presence of a germline configuration of T cell receptor genes. Moreover, an abnormal configuration of the CD8A gene was found in the CD8⁺ B lymphocytic clone. We suggest that the aberrant expression of the CD8 gene could be related to its abnormal configuration.

Original language	English
Pages (from-to)	1544-1550
Number of pages	7
Journal	Leukemia
Volume	10
Issue number	9
Publication status	Published - Sep 1996

Keywords

B-CLL
CD8A gene

ASJC Scopus subject areas

Hematology
Cancer Research

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abstract = "We report the case of a patient with chronic B lymphocytic leukemia, with stable clinical and hematological conditions in the absence of any treatment. The flow cytometry analysis of the patient's cells revealed a CD19+, CD5+, IgM+, IgD+, λ chain+ phenotype, along with an unusual expression of CD8α/α homo dimer. B cells did not stain with the anti CD8β monoclonal antibody T8/2T8 5H7. Molecular biology analyses confirmed the monoclonal rearrangements of immunoglobulin heavy and λ light chain genes in the presence of a germline configuration of T cell receptor genes. Moreover, an abnormal configuration of the CD8A gene was found in the CD8+ B lymphocytic clone. We suggest that the aberrant expression of the CD8 gene could be related to its abnormal configuration.",

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AU - Alosi, M.

AU - Improta, S.

AU - Baccarani, M.

AU - De Paoli, P.

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AB - We report the case of a patient with chronic B lymphocytic leukemia, with stable clinical and hematological conditions in the absence of any treatment. The flow cytometry analysis of the patient's cells revealed a CD19+, CD5+, IgM+, IgD+, λ chain+ phenotype, along with an unusual expression of CD8α/α homo dimer. B cells did not stain with the anti CD8β monoclonal antibody T8/2T8 5H7. Molecular biology analyses confirmed the monoclonal rearrangements of immunoglobulin heavy and λ light chain genes in the presence of a germline configuration of T cell receptor genes. Moreover, an abnormal configuration of the CD8A gene was found in the CD8+ B lymphocytic clone. We suggest that the aberrant expression of the CD8 gene could be related to its abnormal configuration.

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