Immunoradiometric assay of active renin in human plasma: Comparison with plasma renin activity

P. Dessìfulgheri, F. Cocco, N. Glorioso, F. Bandiera, P. Madeddu, A. Rappelli

Research output: Contribution to journalArticlepeer-review

Abstract

The direct measurement of active renin with monoclonal antobodies (IRMA) in plasma from hypertensive patients was compared with the traditional PRA method. Two monoclonal antibodies were used: 3E8 and 4G1. The first was coupled to magnetizable beads and was used to trap both active and inactive renin from plasma. The second antibody, 4G1, was iodinated and used to detect active renin trapped by 3E8. The correlation coefficient between the two methods was very high (r = 0.98, p <0.001) in plasma samples whose PRA values were higher than 2 ng/ml/h; in low renin samples (PRA lower than 2 ng/ml/h) no significant correlation was found (r=0.12 n.s.). When PRA and IRMA were performed before and after trypsin activation of inactive renin, the percentage of inactive over total renin was 86.8% and 84% as calculated with PRA and IRMA respectively. The direct monoclonal antibodies method for measuring active renin can be usefully adopted, in conjunction with the traditional PRA procedure, in studying both clinical and pathophysiological aspects of the renin-angiotensin system.

Original languageEnglish
Pages (from-to)1383-1390
Number of pages8
JournalClinical and Experimental Hypertension
VolumeA9
Issue number8-9
DOIs
Publication statusPublished - 1987

Keywords

  • Immunoradiometric assay
  • Monoclonal antibodies
  • PRA
  • Renin

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology

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