Immunoreactive lactoferrin in resting, activated, and neoplastic lymphocytes

Thomas W. Butler, Carlo E. Grossi, Andrea Canessa, Vito Pistoia, James C. Barton

Research output: Contribution to journalArticlepeer-review


Lactoferrin (Lf) in lymphocytes was assessed with immunofluorescence/flow cytometric technique. Surface Lf was detected primarily among B-cell-enriched preparations. Tonsillar B-cells of different densities expressed surface Lf similarly. Very small percentages of CALLA+ ALL, HCL, or EBV-transformed B-cells expressed surface Lf, whereas B-CLL lymphocytes had the highest percentages of surface Lf positivity. Few resting, cultured, or neoplastic T-lymphocytes expressed Lf. The pattern of immunofluorescence and analyses of surface and total cellular immunoreactive Lf indicated that Lf is associated primarily with the lymphocytes surface. The percentage and/or intensity of surface Lf-specific fluorescence were not significantly altered in B- or T-cells by incubation with physiologic concentrations of diferric Lf, and the percentages of Lf-positive cells detected in respective subjects remained stable over time. Surface Lf positivity was unrelated to the expression of other surface antigens (except those marking B- or T-cell lineage) or cell cycle. Expression and/or binding of Lf in B-lymphocytes may become increased during certain stages of cell maturation.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalLeukemia Research
Issue number5
Publication statusPublished - 1990


  • B-cell
  • B-CLL
  • cell surface
  • Lf
  • lymphocyte
  • T-cell

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology


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