Immunoreactivity for cyclin D3 is frequently detectable in high-grade primary gastric lymphomas in the absence of the t(6,14) (p21.1;q32.3) chromosomal translocation

Giancarlo Pruneri, Sonia Fabris, Roberta Fasani, Barbara Del Curto, Carlo Capella, Barbara Pozzi, Teresio Motta, Salvatore Andreola, Andres J M Ferreri, Maurilio Ponzoni, Giuseppe Viale, Antonio Neri

Research output: Contribution to journalArticle

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Abstract

Cyclin D3 plays a pivotal role in controlling the physiological progression from the G1 to the S phase of the cell cycle. Recent data suggest that cyclin D3 may be deregulated in extranodal non-Hodgkin's lymphomas (NHLs) as a consequence of the t(6;14)(p21.1;q32.3) translocation. The present study investigated for the first time by dual-colour fluorescence in situ hybridization (FISH) on interphase nuclei and immunohistochemistry the prevalence of the t(6;14) translocation and cyclin D3 immunoreactivity (IR) in a series of 29 stage I-IIE primary gastric NHLs (PGLs). No case showed the t(6;14) translocation. However, in five (17.2%) cases (two extranodal marginal zone lymphomas of MALT type, LGM; one diffuse large-cell lymphoma with a MALT component, DLCLM; and two diffuse large-cell lymphomas without a MALT component, DLCL), three to four cyclin D3 signals were detected by FISH. Co-hybridization with probes specific for the centromeric region and long arm of chromosome 6 indicated trisomy in one case (DLCL), whereas in the remaining four cases the pattern was highly suggestive of the presence of an isochromosome 6p. One (12.5%) case of LGM, six (75%) cases of DLCLM, and seven (53.8%) cases of DLCL (p = 0.0378) were immunoreactive for cyclin D3. Cyclin D3 IR was detected in two (40%) of the five cases with extra cyclin D3 signals and in 12 of the remaining 24 cases (50%, p = 1.000). These results suggest that the t(6;14) may represent a rare event in the pathogenesis of PGL and that cyclin D3 deregulation is most likely the result of epigenetic mechanisms.

Original languageEnglish
Pages (from-to)596-601
Number of pages6
JournalJournal of Pathology
Volume200
Issue number5
DOIs
Publication statusPublished - Aug 1 2003

Fingerprint

Cyclin D3
Genetic Translocation
Lymphoma, Large B-Cell, Diffuse
Fluorescence In Situ Hybridization
Non-Hodgkin's Lymphoma
Stomach
Isochromosomes
Marginal Zone B-Cell Lymphoma
Chromosomes, Human, Pair 6
Familial primary gastric lymphoma
Trisomy
Interphase
S Phase
Epigenomics
Cell Cycle
Color
Immunohistochemistry

Keywords

  • Cyclin D3
  • FISH
  • Gastric lymphoma
  • Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunoreactivity for cyclin D3 is frequently detectable in high-grade primary gastric lymphomas in the absence of the t(6,14) (p21.1;q32.3) chromosomal translocation. / Pruneri, Giancarlo; Fabris, Sonia; Fasani, Roberta; Del Curto, Barbara; Capella, Carlo; Pozzi, Barbara; Motta, Teresio; Andreola, Salvatore; Ferreri, Andres J M; Ponzoni, Maurilio; Viale, Giuseppe; Neri, Antonio.

In: Journal of Pathology, Vol. 200, No. 5, 01.08.2003, p. 596-601.

Research output: Contribution to journalArticle

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abstract = "Cyclin D3 plays a pivotal role in controlling the physiological progression from the G1 to the S phase of the cell cycle. Recent data suggest that cyclin D3 may be deregulated in extranodal non-Hodgkin's lymphomas (NHLs) as a consequence of the t(6;14)(p21.1;q32.3) translocation. The present study investigated for the first time by dual-colour fluorescence in situ hybridization (FISH) on interphase nuclei and immunohistochemistry the prevalence of the t(6;14) translocation and cyclin D3 immunoreactivity (IR) in a series of 29 stage I-IIE primary gastric NHLs (PGLs). No case showed the t(6;14) translocation. However, in five (17.2{\%}) cases (two extranodal marginal zone lymphomas of MALT type, LGM; one diffuse large-cell lymphoma with a MALT component, DLCLM; and two diffuse large-cell lymphomas without a MALT component, DLCL), three to four cyclin D3 signals were detected by FISH. Co-hybridization with probes specific for the centromeric region and long arm of chromosome 6 indicated trisomy in one case (DLCL), whereas in the remaining four cases the pattern was highly suggestive of the presence of an isochromosome 6p. One (12.5{\%}) case of LGM, six (75{\%}) cases of DLCLM, and seven (53.8{\%}) cases of DLCL (p = 0.0378) were immunoreactive for cyclin D3. Cyclin D3 IR was detected in two (40{\%}) of the five cases with extra cyclin D3 signals and in 12 of the remaining 24 cases (50{\%}, p = 1.000). These results suggest that the t(6;14) may represent a rare event in the pathogenesis of PGL and that cyclin D3 deregulation is most likely the result of epigenetic mechanisms.",
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AU - Pruneri, Giancarlo

AU - Fabris, Sonia

AU - Fasani, Roberta

AU - Del Curto, Barbara

AU - Capella, Carlo

AU - Pozzi, Barbara

AU - Motta, Teresio

AU - Andreola, Salvatore

AU - Ferreri, Andres J M

AU - Ponzoni, Maurilio

AU - Viale, Giuseppe

AU - Neri, Antonio

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AB - Cyclin D3 plays a pivotal role in controlling the physiological progression from the G1 to the S phase of the cell cycle. Recent data suggest that cyclin D3 may be deregulated in extranodal non-Hodgkin's lymphomas (NHLs) as a consequence of the t(6;14)(p21.1;q32.3) translocation. The present study investigated for the first time by dual-colour fluorescence in situ hybridization (FISH) on interphase nuclei and immunohistochemistry the prevalence of the t(6;14) translocation and cyclin D3 immunoreactivity (IR) in a series of 29 stage I-IIE primary gastric NHLs (PGLs). No case showed the t(6;14) translocation. However, in five (17.2%) cases (two extranodal marginal zone lymphomas of MALT type, LGM; one diffuse large-cell lymphoma with a MALT component, DLCLM; and two diffuse large-cell lymphomas without a MALT component, DLCL), three to four cyclin D3 signals were detected by FISH. Co-hybridization with probes specific for the centromeric region and long arm of chromosome 6 indicated trisomy in one case (DLCL), whereas in the remaining four cases the pattern was highly suggestive of the presence of an isochromosome 6p. One (12.5%) case of LGM, six (75%) cases of DLCLM, and seven (53.8%) cases of DLCL (p = 0.0378) were immunoreactive for cyclin D3. Cyclin D3 IR was detected in two (40%) of the five cases with extra cyclin D3 signals and in 12 of the remaining 24 cases (50%, p = 1.000). These results suggest that the t(6;14) may represent a rare event in the pathogenesis of PGL and that cyclin D3 deregulation is most likely the result of epigenetic mechanisms.

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