Immunoreactivity for latent membrane protein 1 of Epstein-Barr virus in nevi and melanomas is not related to the viral infection

Giovanni Bertalot, Maria Olivia Biasi, Maurizio Gramegna, Jon Askaa, Patrizia Dell'Orto, Giuseppe Viale

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Epstein-Barr virus (EBV) is a human herpes virus with oncogenic potential, associated with several malignancies. The EBV-encoded latent membrane protein 1 (LMP1) is one of nine proteins regularly expressed in virally infected and immortalised B lymphocytes. We now document the consistent immunoreactivity for LMP1 in 90% of 65 nevi and melanomas, using the monoclonal antibody cocktail CS1-4. The immunocytochemical findings, however, were not confirmed using reverse-transcription polymerase chain reaction (RT-PCR) experiments, which failed to demonstrate any actual expression of LMP1 mRNA. In situ hybridisation for EBV-encoded RNAs (EBERs 1 and 2) and PCR amplification of EBV genomic sequences also failed to document any viral infection. Several normal and neoplastic human tissues have also been immunostained for LMP1, without any positive staining, with the exception of a minor percentage of skin melanocytes and of normal blasts of the myeloid and erythroid lineages. We conclude that the vast majority of nevi and melanomas express a still uncharacterised molecule, cross-reacting with anti-LMP1 (CS1-4) antibodies, which may be considered a consistent marker of melanocytic proliferations. The immunoreactivity of normal and neoplastic human tissues for the anti-LMP1 reagent should not be taken as evidence of EBV infection.

Original languageEnglish
Pages (from-to)553-559
Number of pages7
JournalVirchows Archiv
Volume436
Issue number6
DOIs
Publication statusPublished - 2000

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Nevi and Melanomas
Virus Diseases
Membrane Proteins
Human Herpesvirus 4
Polymerase Chain Reaction
Oncogenic Viruses
Epstein-Barr Virus Infections
Melanocytes
Reverse Transcription
In Situ Hybridization
B-Lymphocytes
Monoclonal Antibodies
Epstein-Barr virus EBV-associated membrane antigen
Staining and Labeling
Messenger RNA
Skin
Antibodies
Neoplasms
Proteins

Keywords

  • EBERs
  • Epstein-Barr virus
  • LMP1
  • Melanoma
  • Nevi

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunoreactivity for latent membrane protein 1 of Epstein-Barr virus in nevi and melanomas is not related to the viral infection. / Bertalot, Giovanni; Biasi, Maria Olivia; Gramegna, Maurizio; Askaa, Jon; Dell'Orto, Patrizia; Viale, Giuseppe.

In: Virchows Archiv, Vol. 436, No. 6, 2000, p. 553-559.

Research output: Contribution to journalArticle

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