Immunoregulatory mechanisms of macrophage PPAR-γ in mice with experimental inflammatory bowel disease

R. Hontecillas, W. T. Horne, M. Climent, A. J. Guri, C. Evans, Y. Zhang, B. W. Sobral, J. Bassaganya-Riera

Research output: Contribution to journalArticlepeer-review

Abstract

Peroxisome proliferator-activated receptor-γ (PPAR-γ) is widely expressed in macrophages and has been identified as a putative target for the development of novel therapies against inflammatory bowel disease (IBD). Computational simulations identified macrophages as key targets for therapeutic interventions against IBD. This study aimed to characterize the mechanisms underlying the beneficial effects of macrophage PPAR-γ in IBD. Macrophage-specific PPAR-γ deletion significantly exacerbated clinical activity and colonic pathology, impaired the splenic and mesenteric lymph node regulatory T-cell compartment, increased percentages of lamina propria (LP) CD8+ T cells, increased surface expression of CD40, Ly6C, and Toll-like receptor 4 (TLR-4) in LP macrophages, and upregulated expression of colonic IFN-γ, CXCL9, CXCL10, IL-22, IL1RL1, CCR1, suppressor of cytokine signaling 3, and MHC class II in mice with IBD. Moreover, macrophage PPAR-γ was required for accelerating pioglitazone-mediated recovery from dextran sodium sulfate (DSS) colitis, providing a cellular target for the anti-inflammatory effects of PPAR-γ agonists in IBD.

Original languageEnglish
Pages (from-to)304-313
Number of pages10
JournalMucosal Immunology
Volume4
Issue number3
DOIs
Publication statusPublished - May 2011

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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