Abstract
Several experimental conditions were used in this study to evaluate the in vitro effects of I5-deoxyspergualin on the function of T lymphocytes, B lymphocytes and monocytes from healthy subjects and patients suffering from systemic lupus erythematosus. Whilst the secretion of polyclonal immunoglobulin (Ig) M and IgG from the B lymphocytes of the healthy subjects was diminished by 15-deoxyspergualin, neither the proliferative response of normal T and B cells to mitogenic stimulation nor the cytokine secretory capacity of these cells (e.g. interleukin-2, -4, -6 and γ-interferon) and monocytes (e.g. interleukin-1β and -6) were affected by the drug. In contrast, on the mononuclear cells obtained from the lupus patients not only did 15-deoxyspergualin inhibit the spontaneous production of polyclonal and anti-DNA IgG antibodies but also suppressed interleukin-1β secretion from the monocytes. Other functional responses of T and B cells and monocytes from lupus patients, including mitogenic activation and cytokine secretion, were not altered by the drug. These data suggest that 15-deoxyspergualin possesses a novel mechanism of pharmacological immunosuppression apparently different from that of other immunosuppressants, such as cyclosporin A, FK506 and corticosteroids, that seems to be primarily displayed at the level of autoreactive B cells and monocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 213-220 |
| Number of pages | 8 |
| Journal | European Journal of Pharmacology |
| Volume | 311 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Sep 12 1996 |
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Keywords
- 15-Deoxyspergualin
- B cell
- Cytokine
- Monocyte
- T cell
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Immunosuppressive activity of 15-deoxyspergualin on normal and autoimmune peripheral blood mononuclear cells. / Nicoletti, Ferdinando; Orietta Borghi, M.; Barcellini, Wilma; Fain, Cristina; Beltrami, Barbara; Del Papa, Nicoletta; Schorlemmer, Hans U.; Mottola, Learco; Meroni, Pier Luigi.
In: European Journal of Pharmacology, Vol. 311, No. 2-3, 12.09.1996, p. 213-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunosuppressive activity of 15-deoxyspergualin on normal and autoimmune peripheral blood mononuclear cells
AU - Nicoletti, Ferdinando
AU - Orietta Borghi, M.
AU - Barcellini, Wilma
AU - Fain, Cristina
AU - Beltrami, Barbara
AU - Del Papa, Nicoletta
AU - Schorlemmer, Hans U.
AU - Mottola, Learco
AU - Meroni, Pier Luigi
PY - 1996/9/12
Y1 - 1996/9/12
N2 - Several experimental conditions were used in this study to evaluate the in vitro effects of I5-deoxyspergualin on the function of T lymphocytes, B lymphocytes and monocytes from healthy subjects and patients suffering from systemic lupus erythematosus. Whilst the secretion of polyclonal immunoglobulin (Ig) M and IgG from the B lymphocytes of the healthy subjects was diminished by 15-deoxyspergualin, neither the proliferative response of normal T and B cells to mitogenic stimulation nor the cytokine secretory capacity of these cells (e.g. interleukin-2, -4, -6 and γ-interferon) and monocytes (e.g. interleukin-1β and -6) were affected by the drug. In contrast, on the mononuclear cells obtained from the lupus patients not only did 15-deoxyspergualin inhibit the spontaneous production of polyclonal and anti-DNA IgG antibodies but also suppressed interleukin-1β secretion from the monocytes. Other functional responses of T and B cells and monocytes from lupus patients, including mitogenic activation and cytokine secretion, were not altered by the drug. These data suggest that 15-deoxyspergualin possesses a novel mechanism of pharmacological immunosuppression apparently different from that of other immunosuppressants, such as cyclosporin A, FK506 and corticosteroids, that seems to be primarily displayed at the level of autoreactive B cells and monocytes.
AB - Several experimental conditions were used in this study to evaluate the in vitro effects of I5-deoxyspergualin on the function of T lymphocytes, B lymphocytes and monocytes from healthy subjects and patients suffering from systemic lupus erythematosus. Whilst the secretion of polyclonal immunoglobulin (Ig) M and IgG from the B lymphocytes of the healthy subjects was diminished by 15-deoxyspergualin, neither the proliferative response of normal T and B cells to mitogenic stimulation nor the cytokine secretory capacity of these cells (e.g. interleukin-2, -4, -6 and γ-interferon) and monocytes (e.g. interleukin-1β and -6) were affected by the drug. In contrast, on the mononuclear cells obtained from the lupus patients not only did 15-deoxyspergualin inhibit the spontaneous production of polyclonal and anti-DNA IgG antibodies but also suppressed interleukin-1β secretion from the monocytes. Other functional responses of T and B cells and monocytes from lupus patients, including mitogenic activation and cytokine secretion, were not altered by the drug. These data suggest that 15-deoxyspergualin possesses a novel mechanism of pharmacological immunosuppression apparently different from that of other immunosuppressants, such as cyclosporin A, FK506 and corticosteroids, that seems to be primarily displayed at the level of autoreactive B cells and monocytes.
KW - 15-Deoxyspergualin
KW - B cell
KW - Cytokine
KW - Monocyte
KW - T cell
UR - http://www.scopus.com/inward/record.url?scp=0030581489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030581489&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(96)00409-8
DO - 10.1016/0014-2999(96)00409-8
M3 - Article
C2 - 8891602
AN - SCOPUS:0030581489
VL - 311
SP - 213
EP - 220
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -