Immunotoxin mediated killing of clonable T-lymphocytes infiltrating an irreversibly rejected human renal allograft

S. Siena, M. Bregni, A. Formosa, B. Brando, D. A. Lappi, G. Bonadonna, A. M. Gianni

Research output: Contribution to journalArticlepeer-review

Abstract

We isolated and treated in vitro with a novel CD5-specific saporin immunotoxin, referred to as OKT1-SAP, the cells infiltrating an irreversibly rejected renal allograft from a patient who rejected while on cyclosporine plus steroids and then failed to respond to multiple courses of high-dose steroids, intravenous OKT3 antibody, and local irradiation to the graft. We report here that under experimental conditions achievable in vivo the immunotoxin OKT1-SAP was capable of eliminating in vitro more than 95% of clonable T-lymphocytes infiltrating the rejected allograft of this patient despite their resistance to previous aggressive immunosuppression. To our knowledge, this is the first report of an immunotoxin-mediated suppression of the clonogenic growth of rejected renal allograft infiltrating T-lymphocytes.

Original languageEnglish
Pages (from-to)84-88
Number of pages5
JournalJournal of Biological Regulators and Homeostatic Agents
Volume3
Issue number2
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Endocrinology
  • Physiology
  • Immunology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)

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