Immunotoxins containing saporin 6 and monoclonal antibodies recognizing plasma cell-associated antigens: Effects on target cells and on normal myeloid precursors (CFU-GM)

L. Barbieri, A. Dinota, M. Gobbi, P. L. Tazzari, S. Rizzi, A. Bontadini, R. Massimo Lemoli, S. Tura, F. Stirpe

Research output: Contribution to journalArticlepeer-review

Abstract

Monoclonal antibodies 8A and 62B1, recognizing plasma cell-associated antigens, were covalently linked to saporin 6, a ribosome-inactivating protein similar to the A-chain of ricin. Both immunotoxins were tested on target human cell lines U266 and Raji, on non-target K562 cell line and on myeloid CFU-GM progenitors. The cloning efficiency and viability of target cells were strongly reduced by 8A-saporin 6 and 62B1-saporin 6 immunotoxins, with an ID50 up to 200.000-fold lower than free saporin 6, whilst the K562 non-target cell line was unaffected. Normal human myeloid precursors (CFU-GM) were inhibited by immunotoxins only to a limited extent. An application of this model for autologous bone marrow transplantation in multiple myeloma patients is proposed. Since no eradication of cloning target cells was achieved by a single immunotoxin, mixtures made with different antibodies could help to reach this goal.

Original languageEnglish
Pages (from-to)238-245
Number of pages8
JournalEuropean Journal of Haematology
Volume42
Issue number3
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Hematology

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