In this study we describe immunotoxins prepared with different CD2 monoclonal antibodies (mAbs) and a ribosome-inactivating protein, saporin. The CD2 immunotoxins were tested on different models. Anti-CD2-saporin conjugates inhibited protein synthesis by a neoplastic CD2 + cell line (SKW- 3) and by an interleukin 2 dependent polyclonal CD2 + lymphoid cell culture (T lymphoblasts), with IC50s ranging from 10-13 M to 10-11 M (as saporin). Similar results were obtained with proliferation inhibition tests (3H-thymidine incorporation) on phytohaemagglutinin (PHA) driven lymphoid cultures and on mixed lymphocyte culture activated lymphocytes. Moreover a CD2-ricin A chain conjugate was less effective than an analogous immunotoxin containing the same CD2 mAb and saporin in inhibiting lymphocyte proliferation induced by PHA (IC50 approximately 10-9 M as ricin A chain versus 10-12 M as saporin). The conjugates were not toxic on bone marrow stem cells. These results suggest that CD2-saporin immunotoxins could represent an effective tool for CD2 + lymphomas or leukaemias, and for T- dependent immune disorders, such as transplanted organ rejection and graft- versus-host disease.
|Number of pages||9|
|Journal||British Journal of Haematology|
|Publication status||Published - 1994|
- monoclonal antibodies
ASJC Scopus subject areas