Immunotoxins containing saporin linked to different CD2 monoclonal antibodies: In vitro evaluation

P. L. Tazzari, A. Bolognesi, D. De Totero, R. M. Lemoli, A. Fortuna, R. Conte, M. J. Crumpton, F. Stirpe

Research output: Contribution to journalArticlepeer-review

Abstract

In this study we describe immunotoxins prepared with different CD2 monoclonal antibodies (mAbs) and a ribosome-inactivating protein, saporin. The CD2 immunotoxins were tested on different models. Anti-CD2-saporin conjugates inhibited protein synthesis by a neoplastic CD2 + cell line (SKW- 3) and by an interleukin 2 dependent polyclonal CD2 + lymphoid cell culture (T lymphoblasts), with IC50s ranging from 10-13 M to 10-11 M (as saporin). Similar results were obtained with proliferation inhibition tests (3H-thymidine incorporation) on phytohaemagglutinin (PHA) driven lymphoid cultures and on mixed lymphocyte culture activated lymphocytes. Moreover a CD2-ricin A chain conjugate was less effective than an analogous immunotoxin containing the same CD2 mAb and saporin in inhibiting lymphocyte proliferation induced by PHA (IC50 approximately 10-9 M as ricin A chain versus 10-12 M as saporin). The conjugates were not toxic on bone marrow stem cells. These results suggest that CD2-saporin immunotoxins could represent an effective tool for CD2 + lymphomas or leukaemias, and for T- dependent immune disorders, such as transplanted organ rejection and graft- versus-host disease.

Original languageEnglish
Pages (from-to)97-105
Number of pages9
JournalBritish Journal of Haematology
Volume86
Issue number1
Publication statusPublished - 1994

Keywords

  • CD2
  • immunotoxin
  • monoclonal antibodies
  • saporin

ASJC Scopus subject areas

  • Hematology

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