Abstract
This study aimed to compare the interobserver Cohen κ on H&E staining and on H&E plus p16(INK4a) staining of all cervical biopsy specimens in a population-based screening program. All the colposcopy-guided biopsies generated by the routine screening of 23,258 women aged 25 to 64 years were stained with H&E and H&E plus p16. Biopsy specimens were reviewed by six external experts. The four diagnoses were available in 441 cases. The interobserver κ values were 0.52 (95% confidence interval [CI], 0.45-0.58) and 0.48 (95% CI, 0.42-0.56) with H&E and H&E + p16, respectively, when using a five-group classification (normal, CIN 1, CIN 2, CIN 3, and cancer); adopting a two-group classification (≤CIN 1 and ≥CIN 2), the values were 0.75 (95% CI, 0.66-0.82) and 0.70 (95% CI, 0.61-0.79), respectively. The use of p16 on all cervical biopsy specimens in a screening program showed virtually no effect on reproducibility of the histologic diagnosis.
| Original language | English |
|---|---|
| Title of host publication | American journal of clinical pathology |
| Pages | 367-373 |
| Number of pages | 7 |
| Volume | 141 |
| Edition | 3 |
| DOIs | |
| Publication status | Published - 2014 |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Impact of p16(INK4a) immunohistochemistry staining on interobserver agreement on the diagnosis of cervical intraepithelial neoplasia. / Pacchiarotti, Alberto; Galeotti, Simona; Bellardini, Paola; Chini, Francesco; Collina, Guido; Dalla Palma, Paolo; Ghiringhello, Bruno; Maccallini, Vincenzo; Musolino, Fabio; Negri, Giovanni; Pisa, Roberto; Sabatucci, Ilaria; Giorgi Rossi, Paolo.
American journal of clinical pathology. Vol. 141 3. ed. 2014. p. 367-373.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Impact of p16(INK4a) immunohistochemistry staining on interobserver agreement on the diagnosis of cervical intraepithelial neoplasia.
AU - Pacchiarotti, Alberto
AU - Galeotti, Simona
AU - Bellardini, Paola
AU - Chini, Francesco
AU - Collina, Guido
AU - Dalla Palma, Paolo
AU - Ghiringhello, Bruno
AU - Maccallini, Vincenzo
AU - Musolino, Fabio
AU - Negri, Giovanni
AU - Pisa, Roberto
AU - Sabatucci, Ilaria
AU - Giorgi Rossi, Paolo
PY - 2014
Y1 - 2014
N2 - This study aimed to compare the interobserver Cohen κ on H&E staining and on H&E plus p16(INK4a) staining of all cervical biopsy specimens in a population-based screening program. All the colposcopy-guided biopsies generated by the routine screening of 23,258 women aged 25 to 64 years were stained with H&E and H&E plus p16. Biopsy specimens were reviewed by six external experts. The four diagnoses were available in 441 cases. The interobserver κ values were 0.52 (95% confidence interval [CI], 0.45-0.58) and 0.48 (95% CI, 0.42-0.56) with H&E and H&E + p16, respectively, when using a five-group classification (normal, CIN 1, CIN 2, CIN 3, and cancer); adopting a two-group classification (≤CIN 1 and ≥CIN 2), the values were 0.75 (95% CI, 0.66-0.82) and 0.70 (95% CI, 0.61-0.79), respectively. The use of p16 on all cervical biopsy specimens in a screening program showed virtually no effect on reproducibility of the histologic diagnosis.
AB - This study aimed to compare the interobserver Cohen κ on H&E staining and on H&E plus p16(INK4a) staining of all cervical biopsy specimens in a population-based screening program. All the colposcopy-guided biopsies generated by the routine screening of 23,258 women aged 25 to 64 years were stained with H&E and H&E plus p16. Biopsy specimens were reviewed by six external experts. The four diagnoses were available in 441 cases. The interobserver κ values were 0.52 (95% confidence interval [CI], 0.45-0.58) and 0.48 (95% CI, 0.42-0.56) with H&E and H&E + p16, respectively, when using a five-group classification (normal, CIN 1, CIN 2, CIN 3, and cancer); adopting a two-group classification (≤CIN 1 and ≥CIN 2), the values were 0.75 (95% CI, 0.66-0.82) and 0.70 (95% CI, 0.61-0.79), respectively. The use of p16 on all cervical biopsy specimens in a screening program showed virtually no effect on reproducibility of the histologic diagnosis.
UR - http://www.scopus.com/inward/record.url?scp=84897935210&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897935210&partnerID=8YFLogxK
U2 - 10.1309/AJCPCYWVL61SVKFU
DO - 10.1309/AJCPCYWVL61SVKFU
M3 - Chapter
C2 - 24515764
AN - SCOPUS:84897935210
VL - 141
SP - 367
EP - 373
BT - American journal of clinical pathology
ER -