Impact of p16(INK4a) immunohistochemistry staining on interobserver agreement on the diagnosis of cervical intraepithelial neoplasia.

Alberto Pacchiarotti, Simona Galeotti, Paola Bellardini, Francesco Chini, Guido Collina, Paolo Dalla Palma, Bruno Ghiringhello, Vincenzo Maccallini, Fabio Musolino, Giovanni Negri, Roberto Pisa, Ilaria Sabatucci, Paolo Giorgi Rossi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This study aimed to compare the interobserver Cohen κ on H&E staining and on H&E plus p16(INK4a) staining of all cervical biopsy specimens in a population-based screening program. All the colposcopy-guided biopsies generated by the routine screening of 23,258 women aged 25 to 64 years were stained with H&E and H&E plus p16. Biopsy specimens were reviewed by six external experts. The four diagnoses were available in 441 cases. The interobserver κ values were 0.52 (95% confidence interval [CI], 0.45-0.58) and 0.48 (95% CI, 0.42-0.56) with H&E and H&E + p16, respectively, when using a five-group classification (normal, CIN 1, CIN 2, CIN 3, and cancer); adopting a two-group classification (≤CIN 1 and ≥CIN 2), the values were 0.75 (95% CI, 0.66-0.82) and 0.70 (95% CI, 0.61-0.79), respectively. The use of p16 on all cervical biopsy specimens in a screening program showed virtually no effect on reproducibility of the histologic diagnosis.

Original languageEnglish
Title of host publicationAmerican journal of clinical pathology
Pages367-373
Number of pages7
Volume141
Edition3
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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