Abstract
Background: Storage and shipment conditions of clinical specimens affect the quality of nucleic acids and may interfere with molecular analysis. The aim of our study was to verify whether blood storage at room temperature affects single nucleotide polymorphisms analysis; moreover, we analysed the consequences of serum storage at 4°C on viral load determination of hepatitis B and C viruses. Methods: For single nucleotide polymorphism screening, genomic DNA was extracted from EDTA whole blood samples stored at room temperature for different times, quantified photometrically, and Factor V Leiden point mutation analysis was performed. For viral load determination, serum samples with medium or low viremias were stored at +4°C for different times and analysed by Cobas AmpliPrep/Cobas TaqMan tests for hepatitis B virus (HBV) DNA or hepatitis C virus (HCV) RNA.Results: While mutation analysis was successfully performed on all samples tested, serum storage at +4°C of HBV- and HCV-infected sera decreased viral load, in particular for low viremias.Conclusions: Storage of blood samples at room temperature up to 1 month does not affect the feasibility of genetic analysis, while serum storage at +4°C affects viral load.
| Original language | English |
|---|---|
| Pages (from-to) | 280-282 |
| Number of pages | 3 |
| Journal | Clinical Chemistry and Laboratory Medicine |
| Volume | 46 |
| Issue number | 2 |
| Publication status | Published - 2008 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Impact of storage conditions on genetic analysis or viral load determination in clinical specimens. / Gravina, Paolo; Ciotti, Marco; Masini, Silvia; Valentini, Alessandra; Marcuccilli, Fabbio; Croce, Nicoletta; Federici, Giorgio; Perno, Carlo Federico; Bernardini, Sergio.
In: Clinical Chemistry and Laboratory Medicine, Vol. 46, No. 2, 2008, p. 280-282.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Impact of storage conditions on genetic analysis or viral load determination in clinical specimens.
AU - Gravina, Paolo
AU - Ciotti, Marco
AU - Masini, Silvia
AU - Valentini, Alessandra
AU - Marcuccilli, Fabbio
AU - Croce, Nicoletta
AU - Federici, Giorgio
AU - Perno, Carlo Federico
AU - Bernardini, Sergio
PY - 2008
Y1 - 2008
N2 - Background: Storage and shipment conditions of clinical specimens affect the quality of nucleic acids and may interfere with molecular analysis. The aim of our study was to verify whether blood storage at room temperature affects single nucleotide polymorphisms analysis; moreover, we analysed the consequences of serum storage at 4°C on viral load determination of hepatitis B and C viruses. Methods: For single nucleotide polymorphism screening, genomic DNA was extracted from EDTA whole blood samples stored at room temperature for different times, quantified photometrically, and Factor V Leiden point mutation analysis was performed. For viral load determination, serum samples with medium or low viremias were stored at +4°C for different times and analysed by Cobas AmpliPrep/Cobas TaqMan tests for hepatitis B virus (HBV) DNA or hepatitis C virus (HCV) RNA.Results: While mutation analysis was successfully performed on all samples tested, serum storage at +4°C of HBV- and HCV-infected sera decreased viral load, in particular for low viremias.Conclusions: Storage of blood samples at room temperature up to 1 month does not affect the feasibility of genetic analysis, while serum storage at +4°C affects viral load.
AB - Background: Storage and shipment conditions of clinical specimens affect the quality of nucleic acids and may interfere with molecular analysis. The aim of our study was to verify whether blood storage at room temperature affects single nucleotide polymorphisms analysis; moreover, we analysed the consequences of serum storage at 4°C on viral load determination of hepatitis B and C viruses. Methods: For single nucleotide polymorphism screening, genomic DNA was extracted from EDTA whole blood samples stored at room temperature for different times, quantified photometrically, and Factor V Leiden point mutation analysis was performed. For viral load determination, serum samples with medium or low viremias were stored at +4°C for different times and analysed by Cobas AmpliPrep/Cobas TaqMan tests for hepatitis B virus (HBV) DNA or hepatitis C virus (HCV) RNA.Results: While mutation analysis was successfully performed on all samples tested, serum storage at +4°C of HBV- and HCV-infected sera decreased viral load, in particular for low viremias.Conclusions: Storage of blood samples at room temperature up to 1 month does not affect the feasibility of genetic analysis, while serum storage at +4°C affects viral load.
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M3 - Article
VL - 46
SP - 280
EP - 282
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
SN - 1434-6621
IS - 2
ER -