Abstract
The ability of HCV to mutate in response to cytotoxic T lymphocyte (CTL) pressure is increasingly recognized, but the influence of such a mechanism in viral persistence and final disease outcome has not been ascertained. In this study, we performed a detailed longitudinal analysis of cell mediated immunity and HCV evolution in two self limiting and two chronically evolving HCV acutely infected patients, one of whom transiently controlled viremia. Aminoacid mutations in immunodominant regions of viruses were observed in all patients, although they conferred viral escape from CTL responses only in chronically infected individuals. Resurgence of viremia coincided with the replacement of the original virus quasispecies with mutant viruses that had escaped recognition by primary CD8+ T cell responses and infection persisted in the presence of variant viruses which were less efficiently recognized by preexisting and de novo induced T cell responses.
| Original language | English |
|---|---|
| Pages (from-to) | 398-406 |
| Number of pages | 9 |
| Journal | Virology |
| Volume | 386 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 10 2009 |
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Keywords
- Acute infection
- CD8 T lymphocytes
- HCV
- Viral escape
ASJC Scopus subject areas
- Virology
Cite this
Impact of viral selected mutations on T cell mediated immunity in chronically evolving and self limiting acute HCV infection. / Guglietta, Silvia; Garbuglia, Anna Rosa; Salichos, Leonidas; Ruggeri, Lionello; Folgori, Antonella; Perrone, Maria Paola; Camperio, Cristina; Mellace, Vincenzo; Maio, Giuseppe; Maio, Patrizia; Capobianchi, Maria Rosaria; Spada, Enea; Gargano, Nicola; Scottà, Cristiano; Piccolella, Enza; Del Porto, Paola.
In: Virology, Vol. 386, No. 2, 10.04.2009, p. 398-406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Impact of viral selected mutations on T cell mediated immunity in chronically evolving and self limiting acute HCV infection
AU - Guglietta, Silvia
AU - Garbuglia, Anna Rosa
AU - Salichos, Leonidas
AU - Ruggeri, Lionello
AU - Folgori, Antonella
AU - Perrone, Maria Paola
AU - Camperio, Cristina
AU - Mellace, Vincenzo
AU - Maio, Giuseppe
AU - Maio, Patrizia
AU - Capobianchi, Maria Rosaria
AU - Spada, Enea
AU - Gargano, Nicola
AU - Scottà, Cristiano
AU - Piccolella, Enza
AU - Del Porto, Paola
PY - 2009/4/10
Y1 - 2009/4/10
N2 - The ability of HCV to mutate in response to cytotoxic T lymphocyte (CTL) pressure is increasingly recognized, but the influence of such a mechanism in viral persistence and final disease outcome has not been ascertained. In this study, we performed a detailed longitudinal analysis of cell mediated immunity and HCV evolution in two self limiting and two chronically evolving HCV acutely infected patients, one of whom transiently controlled viremia. Aminoacid mutations in immunodominant regions of viruses were observed in all patients, although they conferred viral escape from CTL responses only in chronically infected individuals. Resurgence of viremia coincided with the replacement of the original virus quasispecies with mutant viruses that had escaped recognition by primary CD8+ T cell responses and infection persisted in the presence of variant viruses which were less efficiently recognized by preexisting and de novo induced T cell responses.
AB - The ability of HCV to mutate in response to cytotoxic T lymphocyte (CTL) pressure is increasingly recognized, but the influence of such a mechanism in viral persistence and final disease outcome has not been ascertained. In this study, we performed a detailed longitudinal analysis of cell mediated immunity and HCV evolution in two self limiting and two chronically evolving HCV acutely infected patients, one of whom transiently controlled viremia. Aminoacid mutations in immunodominant regions of viruses were observed in all patients, although they conferred viral escape from CTL responses only in chronically infected individuals. Resurgence of viremia coincided with the replacement of the original virus quasispecies with mutant viruses that had escaped recognition by primary CD8+ T cell responses and infection persisted in the presence of variant viruses which were less efficiently recognized by preexisting and de novo induced T cell responses.
KW - Acute infection
KW - CD8 T lymphocytes
KW - HCV
KW - Viral escape
UR - http://www.scopus.com/inward/record.url?scp=62949086945&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62949086945&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2009.01.020
DO - 10.1016/j.virol.2009.01.020
M3 - Article
C2 - 19232664
AN - SCOPUS:62949086945
VL - 386
SP - 398
EP - 406
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -