Impaired angiogenic potential of human placental mesenchymal stromal cells in intrauterine growth restriction

Chiara Mandò, Paola Razini, Chiara Novielli, Gaia Maria Anelli, Marzia Belicchi, Silvia Erratico, Stefania Banfi, Mirella Meregalli, Alessandro Tavelli, Marco Baccarin, Alessandro Rolfo, Silvia Motta, Yvan Torrente, Irene Cetin

Research output: Contribution to journalArticle

Abstract

Human placental mesenchymal stromal cells (pMSCs) have never been investigated in intrauterine growth restriction (IUGR). We characterized cells isolated from placental membranes and the basal disc of six IUGR and five physiological placentas. Cell viability and proliferation were assessed every 7 days during a 6-week culture. Expression of hematopoietic, stem, endothelial, and mesenchymal markers was evaluated by flow cytometry. We characterized the multipotency of pMSCs and the expression of genes involved in mitochondrial content and function. Cell viability was high in all samples, and proliferation rate was lower in IUGR compared with control cells. All samples presented a starting heterogeneous population, shifting during culture toward homogeneity for mesenchymal markers and occurring earlier in IUGR than in controls. In vitro multipotency of IUGR-derived pMSCs was restricted because their capacity for adipocyte differentiation was increased, whereas their ability to differentiate toward endothelial cell lineagewasdecreased. Mitochondrial content and function were higher in IUGR pMSCs than controls, possibly indicating a shift from anaerobic to aerobic metabolism, with the loss of the metabolic characteristics that are typical of undifferentiated multipotent cells.

Original languageEnglish
Pages (from-to)451-463
Number of pages13
JournalStem cells translational medicine
Volume5
Issue number4
DOIs
Publication statusPublished - 2016

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Keywords

  • Intrauterine growth restriction
  • Mesenchymal stromal cells
  • Placenta
  • Pregnancy
  • Stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Mandò, C., Razini, P., Novielli, C., Anelli, G. M., Belicchi, M., Erratico, S., Banfi, S., Meregalli, M., Tavelli, A., Baccarin, M., Rolfo, A., Motta, S., Torrente, Y., & Cetin, I. (2016). Impaired angiogenic potential of human placental mesenchymal stromal cells in intrauterine growth restriction. Stem cells translational medicine, 5(4), 451-463. https://doi.org/10.5966/sctm.2015-0155