Impaired apoptosis in mitogen-stimulated lymphocytes of patients with multiple sclerosis

Beatrice Macchi, Claudia Matteucci, Ugo Nocentini, Carlo Caltagirone, Antonio Mastino

Research output: Contribution to journalArticlepeer-review

Abstract

WE investigated the sensitivity to cell death of peripheral blood mononuclear cells (PBMCs) from patients with multiple sclerosis (MS). PBMCs from MS patients, following PHA stimulation, were less sensitive to cell death than those from healthy donors (mean ± s.e.m., 22.5 ± 1.9 in MS patients vs 36.5 ± 2.8 in healthy controls; p= 0.0003). However, when Fas- agonist antibody was added, the increase in respect to apoptosis induced by mitogen alone was even higher in MS patients than in controls. In addition, PHA-activated PBMCs from MS patients showed higher surface expression of Fas than controls, while Bcl-2 expression was decreased. This finding raised the question of whether an impaired generation of apoptotic signals may be contributing to the immune component of MS.

Original languageEnglish
Pages (from-to)399-402
Number of pages4
JournalNeuroReport
Volume10
Issue number2
Publication statusPublished - Feb 5 1999

Keywords

  • Activation-induced cell death
  • Apoptosis
  • Bcl-2
  • Demyelination
  • Fas
  • Multiple sclerosis
  • PHA

ASJC Scopus subject areas

  • Neuroscience(all)

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