Impaired fibrinolysis in retinal vein occlusion: A role for genetic determinants of PAI-I levels

Anna Maria Gori, Rossella Marcucci, Cinzia Fatini, Francesca Gensini, Elena Sticchi, Andrea Sodi, Stefania Cappelli, Ugo Menchini, Gian Franco Gensini, Rosanna Abbate, Domenico Prisco

Research output: Contribution to journalArticle

Abstract

Few and contrasting data are available on the presence of a thrombophilic state in patients with retinal vein occlusion (RVO), and we have previously demonstrated a role of elevated PAI-1 activity as a risk factor for this condition. The present study was undertaken to investigate whether PAI 4G/SG and ACE I/D polymorphisms are independent risk factors for RVO and whether they account for elevated PAI-1 activity levels. We studied 112 RVO patients (52 males and 60 females; range 18-83 years; median age 60 years) and 112 healthy subjects (52 males and 60 females; range 20-84 years; median age 57 years). PAI-1 activity was determined by a chromogenic assay and ACE I/D and PAI-1 4G/5G polymorphisms by polymerase chain reaction (PCR) and restriction length fragment polymorphism (RLFP) methods. Elevated PAI-1 activity (above 95th percentile of the controls) was significantly associated with RVO at multivariate analysis after adjustment for age, sex, traditional cardiovascular risk factors and haemostasis-related risk factors (OR = 4.93, 95% CI 1.70-14.30; p = 0.003). The homozygosity for ACE DD was found to be an independent risk factor for RVO at multivariate analysis (OR = 1.98, 95% CI 1.01-3.83; p = 0.049), whereas no significant association between homozygosity for PAI-1 4G4G and risk of RVO was observed. Subjects carrying both ACE DD genotype and PAI-1 4G4G genotype showed an increased risk for RVO at multivariate analysis (OR = 4.82, 95% CI 1.89-12.29; p = 0.001). In 45/112 patients without the established risk factors for RVO (hypertension, hypercholesterolemia and diabetes) or characteristics known to be associated to increased PAI-1 activity (overweight, hypertriglyceridemia, and smoking habit) the contemporary presence of ACE DD and PAI-1 4G4G genotype was significantly associated with a risk for RVO (OR = 8.26, 95% CI 1.18-57.92; p = 0.034). In conclusion, in our study: I-indicates that ACE DD genotype is a risk factor for RVO in the whole group of patients, and in the subgroup of patients without the established risk factors for RVO or characteristics influencing the PAI-1 activity, when associated to PAI-1 4G4G genotype, and 2-confirms the role of hypofibrinolysis, documented by high levels of PAI-1 activity, in the occurrence of patients with RVO.

Original languageEnglish
Pages (from-to)54-60
Number of pages7
JournalThrombosis and Haemostasis
Volume92
Issue number1
Publication statusPublished - Jul 2004

Keywords

  • Angiotensin converting enzyme (ACE)
  • Genetic polymorphisms
  • Plasminogen activator inhibitor-1 (PAI-1)
  • Retinal vein occlusion
  • Risk factors

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Gori, A. M., Marcucci, R., Fatini, C., Gensini, F., Sticchi, E., Sodi, A., Cappelli, S., Menchini, U., Gensini, G. F., Abbate, R., & Prisco, D. (2004). Impaired fibrinolysis in retinal vein occlusion: A role for genetic determinants of PAI-I levels. Thrombosis and Haemostasis, 92(1), 54-60.